Interstitial engraftment of adipose-derived stem cells into an acellular dermal matrix results in improved inward angiogenesis and tissue incorporation.
Abstract
Acellular dermal matrices (ADM) are commonly used in reconstructive procedures and
rely on host cell invasion to become incorporated into host tissues. We investigated
different approaches to adipose-derived stem cells (ASCs) engraftment into ADM to
enhance this process. Lewis rat adipose-derived stem cells were isolated and grafted
(3.0 × 10(5) cells) to porcine ADM disks (1.5 mm thick × 6 mm diameter) using either
passive onlay or interstitial injection seeding techniques. Following incubation,
seeding efficiency and seeded cell viability were measured in vitro. In addition,
Eighteen Lewis rats underwent subcutaneous placement of ADM disk either as control
or seeded with PKH67 labeled ASCs. ADM disks were seeded with ASCs using either onlay
or injection techniques. On day 7 and or 14, ADM disks were harvested and analyzed
for host cell infiltration. Onlay and injection techniques resulted in unique seeding
patterns; however cell seeding efficiency and cell viability were similar. In-vivo
studies showed significantly increased host cell infiltration towards the ASCs foci
following injection seeding in comparison to control group (p < 0.05). Moreover, regional
endothelial cell invasion was significantly greater in ASCs injected grafts in comparison
to onlay seeding (p < 0.05). ADM can successfully be engrafted with ASCs. Interstitial
engraftment of ASCs into ADM via injection enhances regional infiltration of host
cells and angiogenesis, whereas onlay seeding showed relatively broad and superficial
cell infiltration. These findings may be applied to improve the incorporation of avascular
engineered constructs.
Type
Journal articleSubject
acellular dermal matrixadipose-derived stem cells
angiogenesis
cell engraftment
Acellular Dermis
Adipose Tissue
Animals
Antigens, CD31
Cell Count
Cell Survival
Endothelial Cells
Male
Neovascularization, Physiologic
Prosthesis Implantation
Rats
Rats, Inbred Lew
Stem Cell Transplantation
Stem Cells
Subcutaneous Tissue
Sus scrofa
Permalink
https://hdl.handle.net/10161/10342Published Version (Please cite this version)
10.1002/jbm.a.34582Publication Info
Komatsu, Issei; Yang, Jun; Zhang, Ying; Levin, L Scott; Erdmann, D; Klitzman, Bruce;
& Hollenbeck, Scott T (2013). Interstitial engraftment of adipose-derived stem cells into an acellular dermal matrix
results in improved inward angiogenesis and tissue incorporation. J Biomed Mater Res A, 101(10). pp. 2939-2947. 10.1002/jbm.a.34582. Retrieved from https://hdl.handle.net/10161/10342.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
Collections
More Info
Show full item recordScholars@Duke
Detlev Erdmann
Instructor in the Department of Surgery
Bruce Klitzman
Associate Professor Emeritus in Surgery
Our overriding interests are in the fields of tissue engineering, wound healing, biosensors,
and long term improvement of medical device implantation. My basic research interests
are in the area of physiological mechanisms of optimizing substrate transport to tissue.
This broad topic covers studies on a whole animal, whole organ, hemorheological, microvascular,
cellular, ultrastructural, and molecular level. The current projects include:
1) control of blood flow and flow distribu
Alphabetical list of authors with Scholars@Duke profiles.

Articles written by Duke faculty are made available through the campus open access policy. For more information see: Duke Open Access Policy
Rights for Collection: Scholarly Articles
Works are deposited here by their authors, and represent their research and opinions, not that of Duke University. Some materials and descriptions may include offensive content. More info