Peptide interfacial biomaterials improve endothelial cell adhesion and spreading on synthetic polyglycolic acid materials.
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Resorbable scaffolds such as polyglycolic acid (PGA) are employed in a number of clinical and tissue engineering applications owing to their desirable property of allowing remodeling to form native tissue over time. However, native PGA does not promote endothelial cell adhesion. Here we describe a novel treatment with hetero-bifunctional peptide linkers, termed "interfacial biomaterials" (IFBMs), which are used to alter the surface of PGA to provide appropriate biological cues. IFBMs couple an affinity peptide for the material with a biologically active peptide that promotes desired cellular responses. One such PGA affinity peptide was coupled to the integrin binding domain, Arg-Gly-Asp (RGD), to build a chemically synthesized bimodular 27 amino acid peptide that mediated interactions between PGA and integrin receptors on endothelial cells. Quartz crystal microbalance with dissipation monitoring (QCMD) was used to determine the association constant (K (A) 1 x 10(7) M(-1)) and surface thickness (~3.5 nm). Cell binding studies indicated that IFBM efficiently mediated adhesion, spreading, and cytoskeletal organization of endothelial cells on PGA in an integrin-dependent manner. We show that the IFBM peptide promotes a 200% increase in endothelial cell binding to PGA as well as 70-120% increase in cell spreading from 30 to 60 minutes after plating.
Coated Materials, Biocompatible
Published Version (Please cite this version)10.1007/s10439-010-9986-5
Publication InfoHuang, Xin; Zauscher, Stefan; Klitzman, Bruce; Truskey, George A; Reichert, William M; Kenan, Daniel J; & Grinstaff, Mark W (2010). Peptide interfacial biomaterials improve endothelial cell adhesion and spreading on synthetic polyglycolic acid materials. Ann Biomed Eng, 38(6). pp. 1965-1976. 10.1007/s10439-010-9986-5. Retrieved from https://hdl.handle.net/10161/10344.
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Associate Professor in Surgery
Our overriding interests are in the fields of tissue engineering, wound healing, biosensors, and long term improvement of medical device implantation. My basic research interests are in the area of physiological mechanisms of optimizing substrate transport to tissue. This broad topic covers studies on a whole animal, whole organ, hemorheological, microvascular, cellular, ultrastructural, and molecular level. The current projects include: 1) control of blood flow and flow distribu
Professor Emeritus of Biomedical Engineering
Adjunct Professor of Biomedical Sciences, Makerere University, Kampala, Uganda (pending)Director of the Duke-Makerere BME PartnershipDr. Reichert's research interests have included biosensors, protein mediated cell adhesion, wound healing, and biocompatibilty. Dr. Reichert was the first member of the engineering faculty to receive the Clemson Award from the Society for Biomaterials (there have since been three others) and elected as a Fellow of the International Unio
R. Eugene and Susie E. Goodson Distinguished Professor of Biomedical Engineering
My research interests focus upon the effect of physical forces on the function of vascular cells and skeletal muscle, cell adhesion, and the design of engineered tissues. Current research projects examine the effect of endothelial cell senescence upon permeability to macromolecules and the response to fluid shear stress, the development of microphysiological blood vessels and muscles for evaluation of drug toxicity and the design of engineered endothelialized blood vessels and skelet
Professor in the Department of Mechanical Engineering and Materials Science
My research lies at the intersection of surface and colloid science, polymer materials engineering, and biointerface science, with four central areas of focus: 1. Fabrication, manipulation and characterization of stimulus-responsive biomolecular and bio-inspired polymeric nanostructures on surfaces; 2. Nanotechnology of soft-wet materials and hybrid biological/non-biological microdevices; 3. Receptor-ligand interactions relevant to the diagnostics of infectious diseases; 4. Friction
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