Intravital microscopy evaluation of angiogenesis and its effects on glucose sensor performance.
Abstract
An optical window model for the rodent dorsum was used to perform chronic and quantitative
intravital microscopy and laser Doppler flowmetry of microvascular networks adjacent
to functional and non-functional glucose sensors. The one-sided configuration afforded
direct, real-time observation of the tissue response to bare (unmodified, smooth surface)
sensors and sensors coated with porous poly-L-lactic acid (PLLA). Microvessel length
density and red blood cell flux (blood perfusion) within 1 mm of the sensors were
measured bi-weekly over 2 weeks. When non-functional sensors were fully implanted
beneath the windows, the porous coated sensors had two-fold more vasculature and significantly
higher blood perfusion than bare sensors on Day 14. When functional sensors were implanted
percutaneously, as in clinical use, no differences in baseline current, neovascularization,
or tissue perfusion were observed between bare and porous coated sensors. However,
percutaneously implanted bare sensors had two-fold more vascularity than fully implanted
bare sensors by Day 14, indicating the other factors, such as micromotion, might be
stimulating angiogenesis. Despite increased angiogenesis adjacent to percutaneous
sensors, modest sensor current attenuation occurred over 14 days, suggesting that
factors other than angiogenesis may play a dominant role in determining sensor function.
Type
Journal articleSubject
AnimalsBiosensing Techniques
Blood Glucose
Erythrocytes
Glucose
Lactic Acid
Male
Microscopy
Microvessels
Neovascularization, Pathologic
Perfusion
Polyesters
Polymers
Porosity
Rats
Rats, Sprague-Dawley
Time Factors
Permalink
https://hdl.handle.net/10161/10345Published Version (Please cite this version)
10.1002/jbm.a.32630Publication Info
Koschwanez, HE; Reichert, WM; & Klitzman, B (2010). Intravital microscopy evaluation of angiogenesis and its effects on glucose sensor
performance. J Biomed Mater Res A, 93(4). pp. 1348-1357. 10.1002/jbm.a.32630. Retrieved from https://hdl.handle.net/10161/10345.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
Collections
More Info
Show full item recordScholars@Duke
Bruce Klitzman
Associate Professor Emeritus in Surgery
Our overriding interests are in the fields of tissue engineering, wound healing, biosensors,
and long term improvement of medical device implantation. My basic research interests
are in the area of physiological mechanisms of optimizing substrate transport to tissue.
This broad topic covers studies on a whole animal, whole organ, hemorheological, microvascular,
cellular, ultrastructural, and molecular level. The current projects include:
1) control of blood flow and flow distribu
William M. Reichert
Professor Emeritus of Biomedical Engineering
Adjunct Professor of Biomedical Sciences, Makerere University, Kampala, Uganda (pending)Director
of the Duke-Makerere BME PartnershipDr. Reichert's research interests have included
biosensors, protein mediated cell adhesion, wound healing, and biocompatibilty. Dr.
Reichert was the first member of the engineering faculty to receive the Clemson Award
from the Society for Biomaterials (there have since been three others) and elected
as a Fellow of the International Unio
Alphabetical list of authors with Scholars@Duke profiles.

Articles written by Duke faculty are made available through the campus open access policy. For more information see: Duke Open Access Policy
Rights for Collection: Scholarly Articles
Works are deposited here by their authors, and represent their research and opinions, not that of Duke University. Some materials and descriptions may include offensive content. More info