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Intravital microscopy evaluation of angiogenesis and its effects on glucose sensor performance.

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Date
2010-06-15
Authors
Koschwanez, HE
Reichert, WM
Klitzman, B
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Abstract
An optical window model for the rodent dorsum was used to perform chronic and quantitative intravital microscopy and laser Doppler flowmetry of microvascular networks adjacent to functional and non-functional glucose sensors. The one-sided configuration afforded direct, real-time observation of the tissue response to bare (unmodified, smooth surface) sensors and sensors coated with porous poly-L-lactic acid (PLLA). Microvessel length density and red blood cell flux (blood perfusion) within 1 mm of the sensors were measured bi-weekly over 2 weeks. When non-functional sensors were fully implanted beneath the windows, the porous coated sensors had two-fold more vasculature and significantly higher blood perfusion than bare sensors on Day 14. When functional sensors were implanted percutaneously, as in clinical use, no differences in baseline current, neovascularization, or tissue perfusion were observed between bare and porous coated sensors. However, percutaneously implanted bare sensors had two-fold more vascularity than fully implanted bare sensors by Day 14, indicating the other factors, such as micromotion, might be stimulating angiogenesis. Despite increased angiogenesis adjacent to percutaneous sensors, modest sensor current attenuation occurred over 14 days, suggesting that factors other than angiogenesis may play a dominant role in determining sensor function.
Type
Journal article
Subject
Animals
Biosensing Techniques
Blood Glucose
Erythrocytes
Glucose
Lactic Acid
Male
Microscopy
Microvessels
Neovascularization, Pathologic
Perfusion
Polyesters
Polymers
Porosity
Rats
Rats, Sprague-Dawley
Time Factors
Permalink
https://hdl.handle.net/10161/10345
Published Version (Please cite this version)
10.1002/jbm.a.32630
Publication Info
Koschwanez, HE; Reichert, WM; & Klitzman, B (2010). Intravital microscopy evaluation of angiogenesis and its effects on glucose sensor performance. J Biomed Mater Res A, 93(4). pp. 1348-1357. 10.1002/jbm.a.32630. Retrieved from https://hdl.handle.net/10161/10345.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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Scholars@Duke

Klitzman

Bruce Klitzman

Associate Professor Emeritus in Surgery
Our overriding interests are in the fields of tissue engineering, wound healing, biosensors, and long term improvement of medical device implantation. My basic research interests are in the area of physiological mechanisms of optimizing substrate transport to tissue. This broad topic covers studies on a whole animal, whole organ, hemorheological, microvascular, cellular, ultrastructural, and molecular level. The current projects include: 1) control of blood flow and flow distribu
Reichert

William M. Reichert

Professor Emeritus of Biomedical Engineering
Adjunct Professor of Biomedical Sciences, Makerere University, Kampala, Uganda (pending)Director of the Duke-Makerere BME PartnershipDr. Reichert's research interests have included biosensors, protein mediated cell adhesion, wound healing, and biocompatibilty.  Dr. Reichert was the first member of the engineering faculty to receive the Clemson Award from the Society for Biomaterials (there have since been three others) and elected as a Fellow of the International Unio
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