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A model of sequential heart and composite tissue allotransplant in rats.
Abstract
BACKGROUND: Some of the 600,000 patients with solid organ allotransplants need reconstruction
with a composite tissue allotransplant, such as the hand, abdominal wall, or face.
The aim of this study was to develop a rat model for assessing the effects of a secondary
composite tissue allotransplant on a primary heart allotransplant. METHODS: Hearts
of Wistar Kyoto rats were harvested and transplanted heterotopically to the neck of
recipient Fisher 344 rats. The anastomoses were performed between the donor brachiocephalic
artery and the recipient left common carotid artery, and between the donor pulmonary
artery and the recipient external jugular vein. Recipients received cyclosporine A
for 10 days only. Heart rate was assessed noninvasively. The sequential composite
tissue allotransplant consisted of a 3 x 3-cm abdominal musculocutaneous flap harvested
from Lewis rats and transplanted to the abdomen of the heart allotransplant recipients.
The abdominal flap vessels were connected to the femoral vessels. No further immunosuppression
was administered following the composite tissue allotransplant. Ten days after composite
tissue allotransplantation, rejection of the heart and abdominal flap was assessed
histologically. RESULTS: The rat survival rate of the two-stage transplant surgery
was 80 percent. The transplanted heart rate decreased from 150 +/- 22 beats per minute
immediately after transplant to 83 +/- 12 beats per minute on day 20 (10 days after
stopping immunosuppression). CONCLUSIONS: This sequential allotransplant model is
technically demanding. It will facilitate investigation of the effects of a secondary
composite tissue allotransplant following primary solid organ transplantation and
could be useful in developing future immunotherapeutic strategies.
Type
Journal articleSubject
AnimalsDisease Models, Animal
Graft Rejection
Graft Survival
Heart Rate
Heart Transplantation
Immunosuppressive Agents
Male
Neck
Rats
Rats, Inbred F344
Rats, Inbred WKY
Skin Transplantation
Surgical Flaps
Survival Rate
Thoracic Wall
Transplantation, Heterotopic
Transplantation, Homologous
Treatment Outcome
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https://hdl.handle.net/10161/10346Published Version (Please cite this version)
10.1097/PRS.0b013e3181dbbb64Publication Info
Yang, Jun; Erdmann, Detlev; Chang, JC; Komatsu, Issei; Zhang, YiXin; Wang, DanRu;
... Levin, LS (2010). A model of sequential heart and composite tissue allotransplant in rats. Plast Reconstr Surg, 126(1). pp. 80-86. 10.1097/PRS.0b013e3181dbbb64. Retrieved from https://hdl.handle.net/10161/10346.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Detlev Erdmann
Professor of Surgery
Scott Thomas Hollenbeck
Associate Professor of Surgery
Breast Cancer, Adipose Biology, Lower Extremity Reconstruction
This author no longer has a Scholars@Duke profile, so the information shown here reflects
their Duke status at the time this item was deposited.
Bruce Klitzman
Associate Professor Emeritus in Surgery
Our overriding interests are in the fields of tissue engineering, wound healing, biosensors,
and long term improvement of medical device implantation. My basic research interests
are in the area of physiological mechanisms of optimizing substrate transport to tissue.
This broad topic covers studies on a whole animal, whole organ, hemorheological, microvascular,
cellular, ultrastructural, and molecular level. The current projects include:
1) control of blood flow and flow distribu
Howard Levinson
Associate Professor of Surgery
Fibrosis Wound Healing Cell Contractility Tissue RemodelingMedical device development
including hernia mesh, tissue engineered skin and wound care technologies
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