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    Patient-derived endothelial progenitor cells improve vascular graft patency in a rodent model.

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    1.1 Mb
    Date
    2012-01
    Authors
    Klitzman, Bruce
    Reichert, WM
    Ren, LC
    Stroncek, JD
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    Abstract
    Late outgrowth endothelial progenitor cells (EPCs) derived from the peripheral blood of patients with significant coronary artery disease were sodded into the lumens of small diameter expanded polytetrafluoroethylene (ePTFE) vascular grafts. Grafts (1mm inner diameter) were denucleated and sodded either with native EPCs or with EPCs transfected with an adenoviral vector containing the gene for human thrombomodulin (EPC+AdTM). EPC+AdTM was shown to increase the in vitro rate of graft activated protein C (APC) production 4-fold over grafts sodded with untransfected EPCs (p<0.05). Unsodded control and EPC-sodded and EPC+AdTM-sodded grafts were implanted bilaterally into the femoral arteries of athymic rats for 7 or 28 days. Unsodded control grafts, both with and without denucleation treatment, each exhibited 7 day patency rates of 25%. Unsodded grafts showed extensive thrombosis and were not tested for patency over 28 days. In contrast, grafts sodded with untransfected EPCs or EPC+AdTM both had 7 day patency rates of 88-89% and 28 day patency rates of 75-88%. Intimal hyperplasia was observed near both the proximal and distal anastomoses in all sodded graft conditions but did not appear to be the primary occlusive failure event. This in vivo study suggests autologous EPCs derived from the peripheral blood of patients with coronary artery disease may improve the performance of synthetic vascular grafts, although no differences were observed between untransfected EPCs and TM transfected EPCs.
    Type
    Journal article
    Subject
    Animals
    Blood Vessel Prosthesis
    Cells, Cultured
    Endothelial Cells
    Endothelium, Vascular
    Humans
    Hyperplasia
    Male
    Protein C
    Rats
    Rats, Nude
    Stem Cells
    Tunica Intima
    Vascular Grafting
    Vascular Patency
    Permalink
    https://hdl.handle.net/10161/10349
    Published Version (Please cite this version)
    10.1016/j.actbio.2011.09.002
    Publication Info
    Klitzman, Bruce; Reichert, WM; Ren, LC; & Stroncek, JD (2012). Patient-derived endothelial progenitor cells improve vascular graft patency in a rodent model. Acta Biomater, 8(1). pp. 201-208. 10.1016/j.actbio.2011.09.002. Retrieved from https://hdl.handle.net/10161/10349.
    This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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    Scholars@Duke

    Klitzman

    Bruce Klitzman

    Associate Professor of Surgery
    Our overriding interests are in the fields of tissue engineering, wound healing, biosensors, and long term improvement of medical device implantation. My basic research interests are in the area of physiological mechanisms of optimizing substrate transport to tissue. This broad topic covers studies on a whole animal, whole organ, hemorheological, microvascular, cellular, ultrastructural, and molecular level. The current projects include: 1) control of blood flow and flow distribu
    Open Access

    Articles written by Duke faculty are made available through the campus open access policy. For more information see: Duke Open Access Policy

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