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The evolving capabilities of rhodopsin-based genetically encoded voltage indicators.
Abstract
Protein engineering over the past four years has made rhodopsin-based genetically
encoded voltage indicators a leading candidate to achieve the task of reporting action
potentials from a population of genetically targeted neurons in vivo. Rational design
and large-scale screening efforts have steadily improved the dynamic range and kinetics
of the rhodopsin voltage-sensing domain, and coupling these rhodopsins to bright fluorescent
proteins has supported bright fluorescence readout of the large and rapid rhodopsin
voltage response. The rhodopsin-fluorescent protein fusions have the highest achieved
signal-to-noise ratios for detecting action potentials in neuronal cultures to date,
and have successfully reported single spike events in vivo. Given the rapid pace of
current development, the genetically encoded voltage indicator class is nearing the
goal of robust spike imaging during live-animal behavioral experiments.
Type
Journal articleSubject
Action PotentialsAnimals
Biosensing Techniques
Cells, Cultured
Fluorescence Resonance Energy Transfer
Fluorescent Dyes
Humans
Kinetics
Luminescent Proteins
Neurons
Recombinant Fusion Proteins
Rhodopsin
Voltage-Sensitive Dye Imaging
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https://hdl.handle.net/10161/10439Published Version (Please cite this version)
10.1016/j.cbpa.2015.05.006Publication Info
Gong, Yiyang (2015). The evolving capabilities of rhodopsin-based genetically encoded voltage indicators.
Curr Opin Chem Biol, 27. pp. 84-89. 10.1016/j.cbpa.2015.05.006. Retrieved from https://hdl.handle.net/10161/10439.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Yiyang Gong
Assistant Professor in the Department of Biomedical Engineering

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