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Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum.

dc.contributor.author Friedman, James
dc.contributor.author Alam, S Munir
dc.contributor.author Shen, Xiaoying
dc.contributor.author Xia, Shi-Mao
dc.contributor.author Stewart, Shelley
dc.contributor.author Anasti, Kara
dc.contributor.author Pollara, Justin
dc.contributor.author Fouda, Genevieve G
dc.contributor.author Yang, Guang
dc.contributor.author Kelsoe, Garnett
dc.contributor.author Ferrari, Guido
dc.contributor.author Tomaras, Georgia D
dc.contributor.author Haynes, Barton F
dc.contributor.author Liao, Hua-Xin
dc.contributor.author Moody, M Anthony
dc.contributor.author Permar, Sallie R
dc.coverage.spatial United States
dc.date.accessioned 2015-09-04T17:17:31Z
dc.date.issued 2012
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/22624058
dc.identifier PONE-D-12-07950
dc.identifier.uri https://hdl.handle.net/10161/10587
dc.description.abstract BACKGROUND: Generation of potent anti-HIV antibody responses in mucosal compartments is a potential requirement of a transmission-blocking HIV vaccine. HIV-specific, functional antibody responses are present in breast milk, and these mucosal antibody responses may play a role in protection of the majority of HIV-exposed, breastfeeding infants. Therefore, characterization of HIV-specific antibodies produced by B cells in milk could guide the development of vaccines that elicit protective mucosal antibody responses. METHODS: We isolated B cells from colostrum of an HIV-infected lactating woman with a detectable neutralization response in milk and recombinantly produced and characterized the resulting HIV-1 Envelope (Env)-specific monoclonal antibodies (mAbs). RESULTS: The identified HIV-1 Env-specific colostrum mAbs, CH07 and CH08, represent two of the first mucosally-derived anti-HIV antibodies yet to be reported. Colostrum mAb CH07 is a highly-autoreactive, weakly-neutralizing gp140-specific mAb that binds to linear epitopes in the gp120 C5 region and gp41 fusion domain. In contrast, colostrum mAb CH08 is a nonpolyreactive CD4-inducible (CD4i) gp120-specific mAb with moderate breadth of neutralization. CONCLUSIONS: These novel HIV-neutralizing mAbs isolated from a mucosal compartment provide insight into the ability of mucosal B cell populations to produce functional anti-HIV antibodies that may contribute to protection against virus acquisition at mucosal surfaces.
dc.language eng
dc.publisher Public Library of Science (PLoS)
dc.relation.ispartof PLoS One
dc.relation.isversionof 10.1371/journal.pone.0037648
dc.subject Antibodies, Monoclonal
dc.subject Antibodies, Neutralizing
dc.subject B-Lymphocytes
dc.subject Colostrum
dc.subject Epitopes, B-Lymphocyte
dc.subject Female
dc.subject Fluorescent Antibody Technique, Indirect
dc.subject HIV Antibodies
dc.subject HIV Envelope Protein gp120
dc.subject Humans
dc.subject Neutralization Tests
dc.subject Pregnancy
dc.title Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum.
dc.type Journal article
duke.contributor.id Alam, S Munir|0251502
duke.contributor.id Pollara, Justin|0300144
duke.contributor.id Fouda, Genevieve G|0420439
duke.contributor.id Kelsoe, Garnett|0205291
duke.contributor.id Ferrari, Guido|0108355
duke.contributor.id Tomaras, Georgia D|0204832
duke.contributor.id Haynes, Barton F|0114780
duke.contributor.id Liao, Hua-Xin|0107020
duke.contributor.id Moody, M Anthony|0028821
duke.contributor.id Permar, Sallie R|0554303
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/22624058
pubs.begin-page e37648
pubs.issue 5
pubs.organisational-group Basic Science Departments
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Duke Human Vaccine Institute
pubs.organisational-group Global Health Institute
pubs.organisational-group Immunology
pubs.organisational-group Institutes and Centers
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Duke Human Vaccine Institute
pubs.organisational-group Molecular Genetics and Microbiology
pubs.organisational-group Pathology
pubs.organisational-group Pediatrics
pubs.organisational-group Pediatrics, Infectious Diseases
pubs.organisational-group School of Medicine
pubs.organisational-group Surgery
pubs.organisational-group Surgery, Surgical Sciences
pubs.organisational-group University Institutes and Centers
pubs.publication-status Published
pubs.volume 7
dc.identifier.eissn 1932-6203
duke.contributor.orcid Ferrari, Guido|0000-0001-7747-3349
duke.contributor.orcid Tomaras, Georgia D|0000-0001-8076-1931
duke.contributor.orcid Moody, M Anthony|0000-0002-3890-5855


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