Chlamydia trachomatis Infection Leads to Defined Alterations to the Lipid Droplet Proteome in Epithelial Cells.
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The obligate intracellular bacterium Chlamydia trachomatis is a major human pathogen and a main cause of genital and ocular diseases. During its intracellular cycle, C. trachomatis replicates inside a membrane-bound vacuole termed an "inclusion". Acquisition of lipids (and other nutrients) from the host cell is a critical step in chlamydial replication. Lipid droplets (LD) are ubiquitous, ER-derived neutral lipid-rich storage organelles surrounded by a phospholipids monolayer and associated proteins. Previous studies have shown that LDs accumulate at the periphery of, and eventually translocate into, the chlamydial inclusion. These observations point out to Chlamydia-mediated manipulation of LDs in infected cells, which may impact the function and thereby the protein composition of these organelles. By means of a label-free quantitative mass spectrometry approach we found that the LD proteome is modified in the context of C. trachomatis infection. We determined that LDs isolated from C. trachomatis-infected cells were enriched in proteins related to lipid metabolism, biosynthesis and LD-specific functions. Interestingly, consistent with the observation that LDs intimately associate with the inclusion, a subset of inclusion membrane proteins co-purified with LD protein extracts. Finally, genetic ablation of LDs negatively affected generation of C. trachomatis infectious progeny, consistent with a role for LD biogenesis in optimal chlamydial growth.
Published Version (Please cite this version)10.1371/journal.pone.0124630
Publication InfoSaka, Hector Alex; Thompson, J Will; Chen, Yi-Shan; Dubois, Laura G; Haas, Joel T; Moseley, Arthur; & Valdivia, Raphael H (2015). Chlamydia trachomatis Infection Leads to Defined Alterations to the Lipid Droplet Proteome in Epithelial Cells. PLoS One, 10(4). pp. e0124630. 10.1371/journal.pone.0124630. Retrieved from https://hdl.handle.net/10161/10589.
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Assistant Research Professor of Pharmacology & Cancer Biology
Dr. Thompson's research focuses on the development and deployment of proteomics and metabolomics mass spectrometry techniques for the analysis of biological systems. He is the Assistant Director of the Proteomics and Metabolomics Shared Resource in the Duke School of Medicine. In this role, he enjoys utilizing mass spectrometry 'omics techniques in research collaborations with investigators throughout the Duke community.
Professor of Molecular Genetics and Microbiology
My laboratory is interested in how microbes influence human health, both in the context of host-pathogen and host-commensal interactions. For many pathogens, and certainly for most commensal microbes, it is is poorly understood what is the molecular basis for how host and microbial factors contribute to a beneficial outcome for us. We currently focus on two experimental systems: Chlamydia trachomatis infections are responsible for the bulk of sexually
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