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Chlamydia trachomatis Infection Leads to Defined Alterations to the Lipid Droplet Proteome in Epithelial Cells.

dc.contributor.author Chen, YS
dc.contributor.author Dubois, Laura
dc.contributor.author Haas, JT
dc.contributor.author Moseley, Martin Arthur III
dc.contributor.author Saka, HA
dc.contributor.author Thompson, JW
dc.contributor.author Valdivia, Raphael H
dc.coverage.spatial United States
dc.date.accessioned 2015-09-06T20:42:08Z
dc.date.issued 2015
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/25909443
dc.identifier PONE-D-14-45569
dc.identifier.uri https://hdl.handle.net/10161/10589
dc.description.abstract The obligate intracellular bacterium Chlamydia trachomatis is a major human pathogen and a main cause of genital and ocular diseases. During its intracellular cycle, C. trachomatis replicates inside a membrane-bound vacuole termed an "inclusion". Acquisition of lipids (and other nutrients) from the host cell is a critical step in chlamydial replication. Lipid droplets (LD) are ubiquitous, ER-derived neutral lipid-rich storage organelles surrounded by a phospholipids monolayer and associated proteins. Previous studies have shown that LDs accumulate at the periphery of, and eventually translocate into, the chlamydial inclusion. These observations point out to Chlamydia-mediated manipulation of LDs in infected cells, which may impact the function and thereby the protein composition of these organelles. By means of a label-free quantitative mass spectrometry approach we found that the LD proteome is modified in the context of C. trachomatis infection. We determined that LDs isolated from C. trachomatis-infected cells were enriched in proteins related to lipid metabolism, biosynthesis and LD-specific functions. Interestingly, consistent with the observation that LDs intimately associate with the inclusion, a subset of inclusion membrane proteins co-purified with LD protein extracts. Finally, genetic ablation of LDs negatively affected generation of C. trachomatis infectious progeny, consistent with a role for LD biogenesis in optimal chlamydial growth.
dc.language eng
dc.relation.ispartof PLoS One
dc.relation.isversionof 10.1371/journal.pone.0124630
dc.subject Animals
dc.subject Cell Line
dc.subject Chlamydia Infections
dc.subject Chlamydia trachomatis
dc.subject Epithelial Cells
dc.subject HeLa Cells
dc.subject Humans
dc.subject Lipid Droplets
dc.subject Lipid Metabolism
dc.subject Mice
dc.subject Proteome
dc.subject Proteomics
dc.title Chlamydia trachomatis Infection Leads to Defined Alterations to the Lipid Droplet Proteome in Epithelial Cells.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/25909443
pubs.begin-page e0124630
pubs.issue 4
pubs.organisational-group Basic Science Departments
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Cardiology
pubs.organisational-group Molecular Genetics and Microbiology
pubs.organisational-group Pharmacology & Cancer Biology
pubs.organisational-group School of Medicine
pubs.publication-status Published online
pubs.volume 10
dc.identifier.eissn 1932-6203


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