Show simple item record

Different Mechanisms Confer Gradual Control and Memory at Nutrient- and Stress-Regulated Genes in Yeast.

dc.contributor.author Rienzo, A
dc.contributor.author Poveda-Huertes, D
dc.contributor.author Aydin, S
dc.contributor.author Buchler, NE
dc.contributor.author Pascual-Ahuir, A
dc.contributor.author Proft, M
dc.coverage.spatial United States
dc.date.accessioned 2015-10-02T14:57:19Z
dc.date.issued 2015-11
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/26283730
dc.identifier MCB.00729-15
dc.identifier.uri http://hdl.handle.net/10161/10648
dc.description.abstract Cells respond to environmental stimuli by fine-tuned regulation of gene expression. Here we investigated the dose-dependent modulation of gene expression at high temporal resolution in response to nutrient and stress signals in yeast. The GAL1 activity in cell populations is modulated in a well-defined range of galactose concentrations, correlating with a dynamic change of histone remodeling and RNA polymerase II (RNAPII) association. This behavior is the result of a heterogeneous induction delay caused by decreasing inducer concentrations across the population. Chromatin remodeling appears to be the basis for the dynamic GAL1 expression, because mutants with impaired histone dynamics show severely truncated dose-response profiles. In contrast, the GRE2 promoter operates like a rapid off/on switch in response to increasing osmotic stress, with almost constant expression rates and exclusively temporal regulation of histone remodeling and RNAPII occupancy. The Gal3 inducer and the Hog1 mitogen-activated protein (MAP) kinase seem to determine the different dose-response strategies at the two promoters. Accordingly, GAL1 becomes highly sensitive and dose independent if previously stimulated because of residual Gal3 levels, whereas GRE2 expression diminishes upon repeated stimulation due to acquired stress resistance. Our analysis reveals important differences in the way dynamic signals create dose-sensitive gene expression outputs.
dc.language eng
dc.relation.ispartof Mol Cell Biol
dc.relation.isversionof 10.1128/MCB.00729-15
dc.subject Galactokinase
dc.subject Galactose
dc.subject Gene Expression Regulation, Fungal
dc.subject Genes, Regulator
dc.subject Histones
dc.subject Mitogen-Activated Protein Kinases
dc.subject Oxidoreductases
dc.subject RNA Polymerase II
dc.subject Saccharomyces cerevisiae
dc.subject Saccharomyces cerevisiae Proteins
dc.subject Stress, Physiological
dc.subject Trans-Activators
dc.subject Transcription Factors
dc.title Different Mechanisms Confer Gradual Control and Memory at Nutrient- and Stress-Regulated Genes in Yeast.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/26283730
pubs.begin-page 3669
pubs.end-page 3683
pubs.issue 21
pubs.organisational-group Biology
pubs.organisational-group Duke
pubs.organisational-group Physics
pubs.organisational-group Trinity College of Arts & Sciences
pubs.publication-status Published
pubs.volume 35
dc.identifier.eissn 1098-5549


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record