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Pseudomonas aeruginosa vesicles associate with and are internalized by human lung epithelial cells.

dc.contributor.author Bauman, SJ
dc.contributor.author Kuehn, Margarethe Joanna
dc.coverage.spatial England
dc.date.accessioned 2015-10-05T18:44:01Z
dc.date.issued 2009-02-03
dc.identifier https://www.ncbi.nlm.nih.gov/pubmed/19192306
dc.identifier 1471-2180-9-26
dc.identifier.uri https://hdl.handle.net/10161/10660
dc.description.abstract BACKGROUND: Pseudomonas aeruginosa is the major pathogen associated with chronic and ultimately fatal lung infections in patients with cystic fibrosis (CF). To investigate how P. aeruginosa-derived vesicles may contribute to lung disease, we explored their ability to associate with human lung cells. RESULTS: Purified vesicles associated with lung cells and were internalized in a time- and dose-dependent manner. Vesicles from a CF isolate exhibited a 3- to 4-fold greater association with lung cells than vesicles from the lab strain PAO1. Vesicle internalization was temperature-dependent and was inhibited by hypertonic sucrose and cyclodextrins. Surface-bound vesicles rarely colocalized with clathrin. Internalized vesicles colocalized with the endoplasmic reticulum (ER) marker, TRAPalpha, as well as with ER-localized pools of cholera toxin and transferrin. CF isolates of P. aeruginosa abundantly secrete PaAP (PA2939), an aminopeptidase that associates with the surface of vesicles. Vesicles from a PaAP knockout strain exhibited a 40% decrease in cell association. Likewise, vesicles from PAO1 overexpressing PaAP displayed a significant increase in cell association. CONCLUSION: These data reveal that PaAP promotes the association of vesicles with lung cells. Taken together, these results suggest that P. aeruginosa vesicles can interact with and be internalized by lung epithelial cells and contribute to the inflammatory response during infection.
dc.language eng
dc.relation.ispartof BMC Microbiol
dc.relation.isversionof 10.1186/1471-2180-9-26
dc.subject Aminopeptidases
dc.subject Cell Line
dc.subject Cystic Fibrosis
dc.subject Endoplasmic Reticulum
dc.subject Epithelial Cells
dc.subject Humans
dc.subject Lung
dc.subject Lung Diseases
dc.subject Pseudomonas Infections
dc.subject Temperature
dc.subject Transport Vesicles
dc.title Pseudomonas aeruginosa vesicles associate with and are internalized by human lung epithelial cells.
dc.type Journal article
pubs.author-url https://www.ncbi.nlm.nih.gov/pubmed/19192306
pubs.begin-page 26
pubs.organisational-group Basic Science Departments
pubs.organisational-group Biochemistry
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Molecular Genetics and Microbiology
pubs.organisational-group School of Medicine
pubs.publication-status Published online
pubs.volume 9
dc.identifier.eissn 1471-2180


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