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ARFGAP1 plays a central role in coupling COPI cargo sorting with vesicle formation.

dc.contributor.author Hong, W
dc.contributor.author Hsu, VW
dc.contributor.author Lee, SY
dc.contributor.author Premont, Richard Thomas
dc.contributor.author Yang, J-S
dc.coverage.spatial United States
dc.date.accessioned 2015-10-22T14:20:27Z
dc.date.issued 2005-01-17
dc.identifier https://www.ncbi.nlm.nih.gov/pubmed/15657398
dc.identifier jcb.200404008
dc.identifier.issn 0021-9525
dc.identifier.uri https://hdl.handle.net/10161/10775
dc.description.abstract Examining how key components of coat protein I (COPI) transport participate in cargo sorting, we find that, instead of ADP ribosylation factor 1 (ARF1), its GTPase-activating protein (GAP) plays a direct role in promoting the binding of cargo proteins by coatomer (the core COPI complex). Activated ARF1 binds selectively to SNARE cargo proteins, with this binding likely to represent at least a mechanism by which activated ARF1 is stabilized on Golgi membrane to propagate its effector functions. We also find that the GAP catalytic activity plays a critical role in the formation of COPI vesicles from Golgi membrane, in contrast to the prevailing view that this activity antagonizes vesicle formation. Together, these findings indicate that GAP plays a central role in coupling cargo sorting and vesicle formation, with implications for simplifying models to describe how these two processes are coupled during COPI transport.
dc.language eng
dc.relation.ispartof J Cell Biol
dc.relation.isversionof 10.1083/jcb.200404008
dc.subject ADP-Ribosylation Factor 1
dc.subject ADP-Ribosylation Factors
dc.subject COP-Coated Vesicles
dc.subject Coat Protein Complex I
dc.subject Coatomer Protein
dc.subject GTPase-Activating Proteins
dc.subject Golgi Apparatus
dc.subject Guanosine Triphosphate
dc.subject Intracellular Membranes
dc.subject Mutation
dc.subject Protein Binding
dc.subject Protein Transport
dc.subject Recombinant Proteins
dc.subject SNARE Proteins
dc.subject Transport Vesicles
dc.subject Vesicular Transport Proteins
dc.title ARFGAP1 plays a central role in coupling COPI cargo sorting with vesicle formation.
dc.type Journal article
pubs.author-url https://www.ncbi.nlm.nih.gov/pubmed/15657398
pubs.begin-page 281
pubs.end-page 290
pubs.issue 2
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Institute for Brain Sciences
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Gastroenterology
pubs.organisational-group School of Medicine
pubs.organisational-group University Institutes and Centers
pubs.publication-status Published
pubs.volume 168


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