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A noisy linear map underlies oscillations in cell size and gene expression in bacteria.

dc.contributor.author Buchler, NE
dc.contributor.author Huang, S
dc.contributor.author Pai, Anand
dc.contributor.author Park, H
dc.contributor.author Stamatov, R
dc.contributor.author Tanouchi, Y
dc.contributor.author You, L
dc.coverage.spatial England
dc.date.accessioned 2015-10-29T19:53:34Z
dc.date.issued 2015-07-16
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/26040722
dc.identifier nature14562
dc.identifier.uri http://hdl.handle.net/10161/10801
dc.description.abstract During bacterial growth, a cell approximately doubles in size before division, after which it splits into two daughter cells. This process is subjected to the inherent perturbations of cellular noise and thus requires regulation for cell-size homeostasis. The mechanisms underlying the control and dynamics of cell size remain poorly understood owing to the difficulty in sizing individual bacteria over long periods of time in a high-throughput manner. Here we measure and analyse long-term, single-cell growth and division across different Escherichia coli strains and growth conditions. We show that a subset of cells in a population exhibit transient oscillations in cell size with periods that stretch across several (more than ten) generations. Our analysis reveals that a simple law governing cell-size control-a noisy linear map-explains the origins of these cell-size oscillations across all strains. This noisy linear map implements a negative feedback on cell-size control: a cell with a larger initial size tends to divide earlier, whereas one with a smaller initial size tends to divide later. Combining simulations of cell growth and division with experimental data, we demonstrate that this noisy linear map generates transient oscillations, not just in cell size, but also in constitutive gene expression. Our work provides new insights into the dynamics of bacterial cell-size regulation with implications for the physiological processes involved.
dc.language eng
dc.relation.ispartof Nature
dc.relation.isversionof 10.1038/nature14562
dc.subject Cell Division
dc.subject Cell Size
dc.subject Computer Simulation
dc.subject Escherichia coli
dc.subject Feedback, Physiological
dc.subject Gene Expression Regulation, Bacterial
dc.subject Homeostasis
dc.subject Models, Biological
dc.subject Single-Cell Analysis
dc.subject Time Factors
dc.title A noisy linear map underlies oscillations in cell size and gene expression in bacteria.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/26040722
pubs.begin-page 357
pubs.end-page 360
pubs.issue 7560
pubs.organisational-group Basic Science Departments
pubs.organisational-group Biology
pubs.organisational-group Biomedical Engineering
pubs.organisational-group Duke
pubs.organisational-group Molecular Genetics and Microbiology
pubs.organisational-group Physics
pubs.organisational-group Pratt School of Engineering
pubs.organisational-group School of Medicine
pubs.organisational-group Trinity College of Arts & Sciences
pubs.publication-status Published
pubs.volume 523
dc.identifier.eissn 1476-4687


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