Kinematic and dynamic gait compensations resulting from knee instability in a rat model of osteoarthritis.
Abstract
INTRODUCTION: Osteoarthritis (OA) results in pain and disability; however, preclinical
OA models often focus on joint-level changes. Gait analysis is one method used to
evaluate both preclinical OA models and OA patients. The objective of this study is
to describe spatiotemporal and ground reaction force changes in a rat medial meniscus
transection (MMT) model of knee OA and to compare these gait measures with assays
of weight bearing and tactile allodynia. METHODS: Sixteen rats were used in the study.
The medial collateral ligament (MCL) was transected in twelve Lewis rats (male, 200
to 250 g); in six rats, the medial meniscus was transected, and the remaining six
rats served as sham controls. The remaining four rats served as naïve controls. Gait,
weight-bearing as measured by an incapacitance meter, and tactile allodynia were assessed
on postoperative days 9 to 24. On day 28, knee joints were collected for histology.
Cytokine concentrations in the serum were assessed with a 10-plex cytokine panel.
RESULTS: Weight bearing was not affected by sham or MMT surgery; however, the MMT
group had decreased mechanical paw-withdrawal thresholds in the operated limb relative
to the contralateral limb (P = 0.017). The gait of the MMT group became increasingly
asymmetric from postoperative days 9 to 24 (P = 0.020); moreover, MMT animals tended
to spend more time on their contralateral limb than their operated limb while walking
(P < 0.1). Ground reaction forces confirmed temporal shifts in symmetry and stance
time, as the MMT group had lower vertical and propulsive ground reaction forces in
their operated limb relative to the contralateral limb, naïve, and sham controls (P
< 0.05). Levels of interleukin 6 in the MMT group tended to be higher than naïve controls
(P = 0.072). Histology confirmed increased cartilage damage in the MMT group, consistent
with OA initiation. Post hoc analysis revealed that gait symmetry, stance time imbalance,
peak propulsive force, and serum interleukin 6 concentrations had significant correlations
to the severity of cartilage lesion formation. CONCLUSION: These data indicate significant
gait compensations were present in the MMT group relative to medial collateral ligament
(MCL) injury (sham) alone and naïve controls. Moreover, these data suggest that gait
compensations are likely driven by meniscal instability and/or cartilage damage, and
not by MCL injury alone.
Type
Journal articleSubject
Adaptation, PhysiologicalAnimals
Biomechanical Phenomena
Disease Models, Animal
Gait
Joint Instability
Male
Osteoarthritis, Knee
Rats
Rats, Inbred Lew
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https://hdl.handle.net/10161/10875Published Version (Please cite this version)
10.1186/ar3801Publication Info
Allen, Kyle D; Mata, Brian A; Gabr, Mostafa A; Huebner, Janet L; Adams, Samuel B;
Kraus, Virginia B; ... Setton, Lori A (2012). Kinematic and dynamic gait compensations resulting from knee instability in a rat
model of osteoarthritis. Arthritis Res Ther, 14(2). pp. R78. 10.1186/ar3801. Retrieved from https://hdl.handle.net/10161/10875.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Samuel Bruce Adams Jr.
Associate Professor of Orthopaedic Surgery
Mostafa Gabr
Research Associate, Senior
Dr. Gabr's research has specifically focused on the following broad areas: (i) animal
model of myelopathy, (ii) participating in clinical trials in spine field.In the last
few years, this research agenda has expanded to include collaborative projects and
publications. Dr. Gabr and his colleagues explore benefit of cervical collar following
spine fusion, spinal cord injury model, and transforaminal lumbar interbody fusion.Dr.
Gabr is the author of "Interleukin-17 synergizes with IFNI&
Virginia Byers Kraus
Mary Bernheim Distinguished Professor of Medicine
My special area of expertise is as a clinician scientist investigating osteoarthritis.
Osteoarthritis is the most common form of joint disease in man and its incidence increases
with age. It is a problem of increasing concern to the medical community due to the
increasing longevity of the population. Trained as a molecular biologist and a Rheumatologist,
I endeavor to study this disease from bedside to bench. The work in this laboratory
focuses on osteoarthritis and deals w
Daniel Oliver Schmitt
Professor in the Department of Evolutionary Anthropology
My primary interest is in the evolution of primate locomotion. I am studying the
mechanics of movement in primates and other vertebrates in the laboratory to understand
the relationship between movement and postcranial morphology, and the unique nature
of primates among mammals. Current projects include the origins of primate locomotion
and the evolution of vertebrate bipedalism.
Lori A. Setton
Adjunct Professor of Biomedical Engineering
Research in Setton's laboratory is focused on the role of mechanical factors in the
degeneration and repair of soft tissues of the musculoskeletal system, including the
intervertebral disc, articular cartilage and meniscus. Work in the Laboratory is focused
on engineering and evaluating materials for tissue regeneration and drug delivery.
Studies combining engineering and biology are also used to determine the role of mechanical
factors to promote and control healing of cartilaginous tissues. Re
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