Exercise-induced changes in metabolic intermediates, hormones, and inflammatory markers associated with improvements in insulin sensitivity.
Abstract
OBJECTIVE: To understand relationships between exercise training-mediated improvements
in insulin sensitivity (S(I)) and changes in circulating concentrations of metabolic
intermediates, hormones, and inflammatory mediators. RESEARCH DESIGN AND METHODS:
Targeted mass spectrometry and enzyme-linked immunosorbent assays were used to quantify
metabolic intermediates, hormones, and inflammatory markers at baseline, after 6 months
of exercise training, and 2 weeks after exercise training cessation (n = 53). A principal
components analysis (PCA) strategy was used to relate changes in these intermediates
to changes in S(I). RESULTS: PCA reduced the number of intermediates from 90 to 24
factors composed of biologically related components. With exercise training, improvements
in S(I) were associated with reductions in by-products of fatty acid oxidation and
increases in glycine and proline (P < 0.05, R² = 0.59); these relationships were retained
15 days after cessation of exercise training (P < 0.05, R² = 0.34). CONCLUSIONS: These
observations support prior observations in animal models that exercise training promotes
more efficient mitochondrial β-oxidation and challenges current hypotheses regarding
exercise training and glycine metabolism.
Type
Journal articleSubject
BiomarkersEnzyme-Linked Immunosorbent Assay
Exercise
Female
Hormones
Humans
Inflammation
Male
Middle Aged
Principal Component Analysis
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https://hdl.handle.net/10161/10882Published Version (Please cite this version)
10.2337/dc10-0709Publication Info
Huffman, Kim M; Slentz, Cris A; Bateman, Lori A; Thompson, Dana; Muehlbauer, Michael
J; Bain, James R; ... Kraus, William E (2011). Exercise-induced changes in metabolic intermediates, hormones, and inflammatory markers
associated with improvements in insulin sensitivity. Diabetes Care, 34(1). pp. 174-176. 10.2337/dc10-0709. Retrieved from https://hdl.handle.net/10161/10882.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
James R. Bain
Professor in Medicine
Kim Marie Huffman
Associate Professor of Medicine
Determining the role of physical activity in modulating health outcomes (cardiovascular
disease risk) in persons with rheumatologic diseases (rheumatoid arthritis, gout,
osteoarthritis) Integrating clinical rheumatology, basic immunology, metabolism,
and exercise science in order to reduce morbidity in individuals with arthritis Evaluating
relationships between circulating and intra-muscular metabolic intermediates and insulin
resistance in sedentary as well as individu
Virginia Byers Kraus
Mary Bernheim Distinguished Professor of Medicine
Virginia Byers Kraus, MD, PhD, is the Mary Bernheim Distinguished Professor of Medicine,
Professor of Orthopaedic Surgery, Professor of Pathology and a faculty member of the
Duke Molecular Physiology Institute in the Duke University School of Medicine. She
is a practicing Rheumatologist with over 30 years’ experience in translational musculoskeletal
research focusing on osteoarthritis, the most common of all arthritides. She trained
at Brown University (ScB 1979), Duke University (MD 19
William Erle Kraus
Richard and Pat Johnson University Distinguished Professor
My training, expertise and research interests range from human integrative physiology
and genetics to animal exercise models to cell culture models of skeletal muscle adaptation
to mechanical stretch. I am trained clinically as an internist and preventive cardiologist,
with particular expertise in preventive cardiology and cardiac rehabilitation. My
research training spans molecular biology and cell culture, molecular genetics, and
integrative human exercise physiology and metabolism. I pr
Christopher Bang Newgard
W. David and Sarah W. Stedman Distinguished Professor of Nutrition in the School of
Medicine
Over its 16 year history, our laboratory has investigated mechanisms of metabolic
regulation and fuel homeostasis in mammalian systems. Major projects include: 1)
Mechanisms involved in regulation of insulin secretion from pancreatic islet β-cells
by glucose and other metabolic fuels; 2) Development of methods for protection of
β-cells against immune-mediated damage; 3) Studies on spatial organization and
regulation of systems controlling hepatic glucose balance; 4) Studies
Cris Allan Slentz
Assistant Professor in Medicine
Robert David Stevens
Adjunct Assistant Professor of Medicine
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