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Genotypic analysis of B cell colonies by in situ hybridization. Stoichiometric expression of three VH families in adult C57BL/6 and BALB/c mice.

dc.contributor.author Kelsoe, Garnett
dc.contributor.author Schulze, DH
dc.coverage.spatial United States
dc.date.accessioned 2015-11-18T16:31:51Z
dc.date.issued 1987-07-01
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/3110348
dc.identifier.issn 0022-1007
dc.identifier.uri https://hdl.handle.net/10161/10896
dc.description.abstract The filter paper disc method for cloning inducible lymphocytes was used to census the splenic B cell population of C57BL/6 and BALB/c mice for the expression of three VH gene-families, VH X-24, -Q52, and -J558. B cell colonies, arising from single founder lymphocytes, were identified by in situ hybridization with VH family- and C mu-specific cDNA probes. Some 6.7 X 10(4) C mu+ colonies were screened. Among C57BL/6- or BALB/c-derived colonies, approximately 3% were VH X-24+, approximately 19% were VH Q52+, and approximately 54% were VH J558+. These frequencies are consistent with a process of equiprobable expression for individual VH segments, and provide direct evidence that normal splenic B lymphocytes use a process of random genetic combinatorics to generate the antibody repertoire.
dc.language eng
dc.relation.ispartof J Exp Med
dc.subject Animals
dc.subject Antibody Diversity
dc.subject B-Lymphocytes
dc.subject Cell Division
dc.subject Cell Line
dc.subject DNA
dc.subject Genetic Variation
dc.subject Genotype
dc.subject Immunoglobulin Heavy Chains
dc.subject Immunoglobulin Variable Region
dc.subject Lipopolysaccharides
dc.subject Mice
dc.subject Mice, Inbred BALB C
dc.subject Mice, Inbred C57BL
dc.subject Nucleic Acid Hybridization
dc.subject Spleen
dc.title Genotypic analysis of B cell colonies by in situ hybridization. Stoichiometric expression of three VH families in adult C57BL/6 and BALB/c mice.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/3110348
pubs.begin-page 163
pubs.end-page 172
pubs.issue 1
pubs.organisational-group Basic Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Duke Human Vaccine Institute
pubs.organisational-group Immunology
pubs.organisational-group Institutes and Centers
pubs.organisational-group School of Medicine
pubs.publication-status Published
pubs.volume 166


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