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Very low affinity B cells form germinal centers, become memory B cells, and participate in secondary immune responses when higher affinity competition is reduced.

dc.contributor.author Dal Porto, Joseph M
dc.contributor.author Haberman, AM
dc.contributor.author Kelsoe, Garnett
dc.contributor.author Shlomchik, MJ
dc.coverage.spatial United States
dc.date.accessioned 2015-11-18T16:43:53Z
dc.date.issued 2002-05-06
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/11994427
dc.identifier.issn 0022-1007
dc.identifier.uri https://hdl.handle.net/10161/10908
dc.description.abstract To understand the relationship between the affinity of the B cell antigen receptor (BCR) and the immune response to antigen, two lines of immunoglobulin H chain transgenic (Tg) mice were created. H50Gmu(a) and T1(V23)mu(a) mice express mu H chain transgenes that associate with the lambda1 L chains to bind the (4-hydroxy-3-nitrophenyl)acetyl hapten with association constants (K(a)s) of only 1.2 x 10(5) M(-1) and 3 x 10(4) M(-1), respectively. Both lines mounted substantial antibody-forming cell (AFC) and germinal center (GC) responses. H50Gmu(a) Tg mice also generated memory B cells. T1(V23)mu(a) B cells formed AFC and GCs, but were largely replaced in late GCs by antigen-specific cells that express endogenous BCRs. Thus, B lymphocytes carrying BCRs with affinities previously thought to be irrelevant in specific immune responses are in fact capable of complete T cell-dependent immune responses when relieved of substantial competition from other B cells. The failure to observe such B cells normally in late primary responses and in memory B cell populations is the result of competition, rather than an intrinsic inability of low affinity B cells.
dc.language eng
dc.relation.ispartof J Exp Med
dc.subject Animals
dc.subject Antibody Formation
dc.subject B-Lymphocytes
dc.subject Gene Rearrangement, B-Lymphocyte
dc.subject Immunoglobulin Heavy Chains
dc.subject Immunologic Memory
dc.subject Mice
dc.subject Mice, SCID
dc.subject Mice, Transgenic
dc.subject Receptors, Antigen, B-Cell
dc.subject T-Lymphocytes
dc.title Very low affinity B cells form germinal centers, become memory B cells, and participate in secondary immune responses when higher affinity competition is reduced.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/11994427
pubs.begin-page 1215
pubs.end-page 1221
pubs.issue 9
pubs.organisational-group Basic Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Duke Human Vaccine Institute
pubs.organisational-group Immunology
pubs.organisational-group Institutes and Centers
pubs.organisational-group School of Medicine
pubs.publication-status Published
pubs.volume 195


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