Developmental mechanism of the periodic membrane skeleton in axons.
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Actin, spectrin, and associated molecules form a periodic sub-membrane lattice structure in axons. How this membrane skeleton is developed and why it preferentially forms in axons are unknown. Here, we studied the developmental mechanism of this lattice structure. We found that this structure emerged early during axon development and propagated from proximal regions to distal ends of axons. Components of the axon initial segment were recruited to the lattice late during development. Formation of the lattice was regulated by the local concentration of βII spectrin, which is higher in axons than in dendrites. Increasing the dendritic concentration of βII spectrin by overexpression or by knocking out ankyrin B induced the formation of the periodic structure in dendrites, demonstrating that the spectrin concentration is a key determinant in the preferential development of this structure in axons and that ankyrin B is critical for the polarized distribution of βII spectrin in neurites.
Published Version (Please cite this version)10.7554/eLife.04581
Publication InfoZhong, Guisheng; He, Jiang; Zhou, Ruobo; Lorenzo, Damaris; Babcock, Hazen P; Bennett, Vann; & Zhuang, Xiaowei (2014). Developmental mechanism of the periodic membrane skeleton in axons. Elife, 3. 10.7554/eLife.04581. Retrieved from https://hdl.handle.net/10161/10975.
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George Barth Geller Distinguished Professor of Molecular Biology
A major interest of our laboratory is in understanding how cells in metazoan organisms manage to target ion channels to physiological sites that optimize their physiological efficiency. Our research began with discovery of the ankyrin family of membrane-adapter proteins, which interact with structurally diverse membrane proteins and couple these proteins to the spectrin-based membrane skeleton. Currently identified ankyrin partners are anion exchangers, the Na/K ATPase, the voltage-depend