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Cytotoxicity of alpha-particle-emitting astatine-211-labelled antibody in tumour spheroids: no effect of hyperthermia.

dc.contributor.author Hauck, ML
dc.contributor.author Larsen, RH
dc.contributor.author Welsh, PC
dc.contributor.author Zalutsky, Michael Rod
dc.coverage.spatial England
dc.date.accessioned 2015-12-03T18:27:54Z
dc.date.issued 1998-03
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/9514054
dc.identifier.issn 0007-0920
dc.identifier.uri http://hdl.handle.net/10161/11043
dc.description.abstract The high linear energy transfer, alpha-particle-emitting radionuclide astatine-211 (211At) is of interest for certain therapeutic applications; however, because of the 55- to 70-microm path length of its alpha-particles, achieving homogeneous tracer distribution is critical. Hyperthermia may enhance the therapeutic efficacy of alpha-particle endoradiotherapy if it can improve tracer distribution. In this study, we have investigated whether hyperthermia increased the cytotoxicity of an 211At-labelled monoclonal antibody (MAb) in tumour spheroids with a radius (approximately 100 microm) greater than the range of 211At alpha-particles. Hyperthermia for 1 h at 42 degrees C was used because this treatment itself resulted in no regrowth delay. Radiolabelled chimeric MAb 81C6 reactive with the extracellular matrix antigen tenascin was added to spheroids grown from the D-247 MG human glioma cell line at activity concentrations ranging from 0.125 to 250 kBq ml(-1). A significant regrowth delay was observed at 125 and 250 kBq ml(-1) in both hyperthermia-treated and untreated spheroids. For groups receiving hyperthermia, no increase in cytotoxicity was seen compared with normothermic controls at any activity concentration. These results and those from autoradiographs indicate that hyperthermia at 42 degrees C for 1 h had no significant effect on the uptake or distribution of this antitenascin MAb in D-247 MG spheroids.
dc.language eng
dc.relation.ispartof Br J Cancer
dc.subject Alpha Particles
dc.subject Animals
dc.subject Antibodies, Monoclonal
dc.subject Antibody Specificity
dc.subject Astatine
dc.subject Biological Transport
dc.subject Gliosarcoma
dc.subject Humans
dc.subject Hyperthermia, Induced
dc.subject Immunoglobulin G
dc.subject Mice
dc.subject Organoids
dc.subject Radioimmunotherapy
dc.subject Recombinant Fusion Proteins
dc.subject Tenascin
dc.subject Tumor Cells, Cultured
dc.title Cytotoxicity of alpha-particle-emitting astatine-211-labelled antibody in tumour spheroids: no effect of hyperthermia.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/9514054
pubs.begin-page 753
pubs.end-page 759
pubs.issue 5
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Pathology
pubs.organisational-group Radiation Oncology
pubs.organisational-group Radiology
pubs.organisational-group School of Medicine
pubs.publication-status Published
pubs.volume 77


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