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Comparative analyses of clinical and environmental populations of Cryptococcus neoformans in Botswana.

dc.contributor.author Chen, Y
dc.contributor.author Fang, C
dc.contributor.author Frazzitta, AE
dc.contributor.author Haverkamp, MR
dc.contributor.author Litvintseva, AP
dc.contributor.author Mitchell, Thomas Greenfield
dc.contributor.author Muthoga, C
dc.contributor.author Perfect, John Robert
dc.contributor.author Wang, L
dc.coverage.spatial England
dc.date.accessioned 2015-12-03T18:56:23Z
dc.date.issued 2015-07
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/26053414
dc.identifier.uri http://hdl.handle.net/10161/11053
dc.description.abstract Cryptococcus neoformans var. grubii (Cng) is the most common cause of fungal meningitis, and its prevalence is highest in sub-Saharan Africa. Patients become infected by inhaling airborne spores or desiccated yeast cells from the environment, where the fungus thrives in avian droppings, trees and soil. To investigate the prevalence and population structure of Cng in southern Africa, we analysed isolates from 77 environmental samples and 64 patients. We detected significant genetic diversity among isolates and strong evidence of geographic structure at the local level. High proportions of isolates with the rare MATa allele were observed in both clinical and environmental isolates; however, the mating-type alleles were unevenly distributed among different subpopulations. Nearly equal proportions of the MATa and MATα mating types were observed among all clinical isolates and in one environmental subpopulation from the eastern part of Botswana. As previously reported, there was evidence of both clonality and recombination in different geographic areas. These results provide a foundation for subsequent genomewide association studies to identify genes and genotypes linked to pathogenicity in humans.
dc.language eng
dc.relation.ispartof Mol Ecol
dc.relation.isversionof 10.1111/mec.13260
dc.subject Cryptococcus neoformans
dc.subject genotyping
dc.subject population structure
dc.subject recombination
dc.subject Alleles
dc.subject Botswana
dc.subject Cryptococcosis
dc.subject Cryptococcus neoformans
dc.subject DNA, Fungal
dc.subject Environment
dc.subject Genes, Mating Type, Fungal
dc.subject Genetic Variation
dc.subject Genetics, Population
dc.subject Geography
dc.subject Humans
dc.subject Molecular Sequence Data
dc.subject Multilocus Sequence Typing
dc.subject Sequence Analysis, DNA
dc.subject Trees
dc.title Comparative analyses of clinical and environmental populations of Cryptococcus neoformans in Botswana.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/26053414
pubs.begin-page 3559
pubs.end-page 3571
pubs.issue 14
pubs.organisational-group Basic Science Departments
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Infectious Diseases
pubs.organisational-group Molecular Genetics and Microbiology
pubs.organisational-group School of Medicine
pubs.publication-status Published
pubs.volume 24
dc.identifier.eissn 1365-294X


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