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Microfluidic platform versus conventional real-time polymerase chain reaction for the detection of Mycoplasma pneumoniae in respiratory specimens. Wulff-Burchfield, Elizabeth Schell, Wiley A Eckhardt, Allen E Pollack, Michael G Hua, Zhishan Rouse, Jeremy L Pamula, Vamsee K Srinivasan, Vijay Benton, Jonathan L Alexander, Barbara D Wilfret, David A Kraft, Monica Cairns, Charles B Perfect, John R Mitchell, Thomas G
dc.coverage.spatial United States 2015-12-03T21:07:25Z 2010-05
dc.identifier S0732-8893(09)00511-2
dc.description.abstract Rapid, accurate diagnosis of community-acquired pneumonia (CAP) due to Mycoplasma pneumoniae is compromised by low sensitivity of culture and serology. Polymerase chain reaction (PCR) has emerged as a sensitive method to detect M. pneumoniae DNA in clinical specimens. However, conventional real-time PCR is not cost-effective for routine or outpatient implementation. Here, we evaluate a novel microfluidic real-time PCR platform (Advanced Liquid Logic, Research Triangle Park, NC) that is rapid, portable, and fully automated. We enrolled patients with CAP and extracted DNA from nasopharyngeal wash (NPW) specimens using a biotinylated capture probe and streptavidin-coupled magnetic beads. Each extract was tested for M. pneumoniae-specific DNA by real-time PCR on both conventional and microfluidic platforms using Taqman probe and primers. Three of 59 (5.0%) NPWs were positive, and agreement between the methods was 98%. The microfluidic platform was equally sensitive but 3 times faster and offers an inexpensive and convenient diagnostic test for microbial DNA.
dc.language eng
dc.publisher Elsevier BV
dc.relation.ispartof Diagn Microbiol Infect Dis
dc.relation.isversionof 10.1016/j.diagmicrobio.2009.12.020
dc.subject Automation
dc.subject Bacteriological Techniques
dc.subject Community-Acquired Infections
dc.subject Humans
dc.subject Microfluidics
dc.subject Molecular Diagnostic Techniques
dc.subject Mycoplasma pneumoniae
dc.subject Nasopharynx
dc.subject Pneumonia, Mycoplasma
dc.subject Point-of-Care Systems
dc.subject Polymerase Chain Reaction
dc.subject Sensitivity and Specificity
dc.subject Time Factors
dc.title Microfluidic platform versus conventional real-time polymerase chain reaction for the detection of Mycoplasma pneumoniae in respiratory specimens.
dc.type Journal article Schell, Wiley A|0064474 Alexander, Barbara D|0071949 Kraft, Monica|0330698 Perfect, John R|0113110 Mitchell, Thomas G|0115609
pubs.begin-page 22
pubs.end-page 29
pubs.issue 1
pubs.organisational-group Basic Science Departments
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Infectious Diseases
pubs.organisational-group Medicine, Pulmonary, Allergy, and Critical Care Medicine
pubs.organisational-group Molecular Genetics and Microbiology
pubs.organisational-group Pathology
pubs.organisational-group School of Medicine
pubs.publication-status Published
pubs.volume 67
dc.identifier.eissn 1879-0070
duke.contributor.orcid Alexander, Barbara D|0000-0001-5868-0529
duke.contributor.orcid Perfect, John R|0000-0002-6606-9460|0000-0003-3465-5518

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