Maintenance and neuronal differentiation of chicken induced pluripotent stem-like cells.
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Pluripotent stem cells have the potential to become any cell in the adult body, including neurons and glia. Avian stem cells could be used to study questions, like vocal learning, that would be difficult to examine with traditional mouse models. Induced pluripotent stem cells (iPSCs) are differentiated cells that have been reprogrammed to a pluripotent stem cell state, usually using inducing genes or other molecules. We recently succeeded in generating avian iPSC-like cells using mammalian genes, overcoming a limitation in the generation and use of iPSCs in nonmammalian species (Rosselló et al., 2013). However, there were no established optimal cell culture conditions for avian iPSCs to establish long-term cell lines and thus to study neuronal differentiation in vitro. Here we present an efficient method of maintaining chicken iPSC-like cells and for differentiating them into action potential generating neurons.
Published Version (Please cite this version)10.1155/2014/182737
Publication InfoChen, CC; Dai, R; Davison, I; Hochgeschwender, U; Jarvis, Erich David; Kessler, J; & Rossello, R (2014). Maintenance and neuronal differentiation of chicken induced pluripotent stem-like cells. Stem Cells Int, 2014. pp. 182737. 10.1155/2014/182737. Retrieved from http://hdl.handle.net/10161/11143.
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Adjunct Professor in the Dept. of Neurobiology
Dr. Jarvis' laboratory studies the neurobiology of vocal communication. Emphasis is placed on the molecular pathways involved in the perception and production of learned vocalizations. They use an integrative approach that combines behavioral, anatomical, electrophysiological and molecular biological techniques. The main animal model used is songbirds, one of the few vertebrate groups that evolved the ability to learn vocalizations. The generality of the discoveries is tested in other vocal