Maintenance and neuronal differentiation of chicken induced pluripotent stem-like cells.
Abstract
Pluripotent stem cells have the potential to become any cell in the adult body, including
neurons and glia. Avian stem cells could be used to study questions, like vocal learning,
that would be difficult to examine with traditional mouse models. Induced pluripotent
stem cells (iPSCs) are differentiated cells that have been reprogrammed to a pluripotent
stem cell state, usually using inducing genes or other molecules. We recently succeeded
in generating avian iPSC-like cells using mammalian genes, overcoming a limitation
in the generation and use of iPSCs in nonmammalian species (Rosselló et al., 2013).
However, there were no established optimal cell culture conditions for avian iPSCs
to establish long-term cell lines and thus to study neuronal differentiation in vitro.
Here we present an efficient method of maintaining chicken iPSC-like cells and for
differentiating them into action potential generating neurons.
Type
Journal articlePermalink
https://hdl.handle.net/10161/11143Published Version (Please cite this version)
10.1155/2014/182737Publication Info
Dai, Rui; Rossello, Ricardo; Chen, Chun-Chun; Kessler, Joeran; Davison, Ian; Hochgeschwender,
Ute; & Jarvis, Erich D (2014). Maintenance and neuronal differentiation of chicken induced pluripotent stem-like
cells. Stem Cells Int, 2014. pp. 182737. 10.1155/2014/182737. Retrieved from https://hdl.handle.net/10161/11143.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
Collections
More Info
Show full item recordScholars@Duke
Erich David Jarvis
Adjunct Professor in the Deptartment of Neurobiology
Dr. Jarvis' laboratory studies the neurobiology of vocal communication. Emphasis is
placed on the molecular pathways involved in the perception and production of learned
vocalizations. They use an integrative approach that combines behavioral, anatomical,
electrophysiological and molecular biological techniques. The main animal model used
is songbirds, one of the few vertebrate groups that evolved the ability to learn vocalizations.
The generality of the discoveries is tested in other vocal lear

Articles written by Duke faculty are made available through the campus open access policy. For more information see: Duke Open Access Policy
Rights for Collection: Scholarly Articles
Works are deposited here by their authors, and represent their research and opinions, not that of Duke University. Some materials and descriptions may include offensive content. More info