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Maintenance and neuronal differentiation of chicken induced pluripotent stem-like cells.

dc.contributor.author Chen, CC
dc.contributor.author Dai, R
dc.contributor.author Davison, I
dc.contributor.author Hochgeschwender, U
dc.contributor.author Jarvis, Erich David
dc.contributor.author Kessler, J
dc.contributor.author Rossello, R
dc.coverage.spatial United States
dc.date.accessioned 2015-12-11T01:32:43Z
dc.date.issued 2014
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/25610469
dc.identifier.issn 1687-966X
dc.identifier.uri https://hdl.handle.net/10161/11143
dc.description.abstract Pluripotent stem cells have the potential to become any cell in the adult body, including neurons and glia. Avian stem cells could be used to study questions, like vocal learning, that would be difficult to examine with traditional mouse models. Induced pluripotent stem cells (iPSCs) are differentiated cells that have been reprogrammed to a pluripotent stem cell state, usually using inducing genes or other molecules. We recently succeeded in generating avian iPSC-like cells using mammalian genes, overcoming a limitation in the generation and use of iPSCs in nonmammalian species (Rosselló et al., 2013). However, there were no established optimal cell culture conditions for avian iPSCs to establish long-term cell lines and thus to study neuronal differentiation in vitro. Here we present an efficient method of maintaining chicken iPSC-like cells and for differentiating them into action potential generating neurons.
dc.language eng
dc.relation.ispartof Stem Cells Int
dc.relation.isversionof 10.1155/2014/182737
dc.title Maintenance and neuronal differentiation of chicken induced pluripotent stem-like cells.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/25610469
pubs.begin-page 182737
pubs.organisational-group Basic Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Institute for Brain Sciences
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Neurobiology
pubs.organisational-group School of Medicine
pubs.organisational-group University Institutes and Centers
pubs.publication-status Published
pubs.volume 2014


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