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A membrane-associated progesterone-binding protein, 25-Dx, is regulated by progesterone in brain regions involved in female reproductive behaviors.

dc.contributor.author Chan, Johnny
dc.contributor.author Jarvis, Erich David
dc.contributor.author Krebs, CJ
dc.contributor.author Lydon, JP
dc.contributor.author Ogawa, S
dc.contributor.author Pfaff, DW
dc.coverage.spatial United States
dc.date.accessioned 2015-12-19T13:50:56Z
dc.date.issued 2000-11-07
dc.identifier https://www.ncbi.nlm.nih.gov/pubmed/11070092
dc.identifier 97/23/12816
dc.identifier.issn 0027-8424
dc.identifier.uri http://hdl.handle.net/10161/11216
dc.description.abstract The ventromedial hypothalamus (VMH) plays a central role in the regulation of the female reproductive behavior lordosis, a behavior dependent upon the sequential activation of receptors for the ovarian steroid hormones estradiol (E) and progesterone (P). These receptors function as transcription factors to alter the expression of target genes. To discover behaviorally relevant genes targeted by E and P in the VMH, we used the differential display PCR to identify messenger RNAs that are differentially expressed in the hypothalamus of ovariectomized (ovx) rats treated with E alone compared with ovariectomized rats treated with E and P. We show here that one interesting mRNA within the hypothalamus that is repressed by P after E priming encodes the protein 25-Dx, the rat homolog of the human membrane-associated P-binding protein Hpr6.6. Neurons in the brain containing the highest levels of 25-Dx are located in several nuclei of the basal forebrain, including the VMH. 25-Dx expression is also higher in the hypothalamus of female P receptor "knockout" mice than in their wild-type littermates. These findings suggest a mechanism in which the activation of nuclear P receptor represses expression of a membrane P receptor, 25-Dx, during lordosis facilitation.
dc.language eng
dc.relation.ispartof Proc Natl Acad Sci U S A
dc.relation.isversionof 10.1073/pnas.97.23.12816
dc.subject Animals
dc.subject Base Sequence
dc.subject Brain
dc.subject Cell Membrane
dc.subject DNA, Complementary
dc.subject Estradiol
dc.subject Female
dc.subject Gene Expression
dc.subject Hypothalamus
dc.subject Membrane Proteins
dc.subject Mice
dc.subject Mice, Knockout
dc.subject Molecular Sequence Data
dc.subject Neurons
dc.subject Neurosecretory Systems
dc.subject Posture
dc.subject Progesterone
dc.subject Rats
dc.subject Rats, Sprague-Dawley
dc.subject Receptors, Progesterone
dc.subject Reproduction
dc.subject Sex Characteristics
dc.subject Sexual Behavior, Animal
dc.title A membrane-associated progesterone-binding protein, 25-Dx, is regulated by progesterone in brain regions involved in female reproductive behaviors.
dc.type Journal article
pubs.author-url https://www.ncbi.nlm.nih.gov/pubmed/11070092
pubs.begin-page 12816
pubs.end-page 12821
pubs.issue 23
pubs.organisational-group Basic Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Institute for Brain Sciences
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Neurobiology
pubs.organisational-group School of Medicine
pubs.organisational-group University Institutes and Centers
pubs.publication-status Published
pubs.volume 97


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