High-resolution reconstruction of fluorescent inclusions in mouse thorax using anatomically guided sampling and parallel Monte Carlo computing.
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We present a method for high-resolution reconstruction of fluorescent images of the mouse thorax. It features an anatomically guided sampling method to retrospectively eliminate problematic data and a parallel Monte Carlo software package to compute the Jacobian matrix for the inverse problem. The proposed method was capable of resolving microliter-sized femtomole amount of quantum dot inclusions closely located in the middle of the mouse thorax. The reconstruction was verified against co-registered micro-CT data. Using the proposed method, the new system achieved significantly higher resolution and sensitivity compared to our previous system consisting of the same hardware. This method can be applied to any system utilizing similar imaging principles to improve imaging performance.
Subject(110.0113) Imaging through turbid media
(170.3010) Image reconstruction techniques
(170.3880) Medical and biological imaging
Published Version (Please cite this version)10.1364/BOE.2.002449
Publication InfoZhang, Xiaofeng; Badea, Cristian; Hood, Greg; Wetzel, Arthur; Qi, Yi; Stiles, Joel; & Johnson, G Allan (2011). High-resolution reconstruction of fluorescent inclusions in mouse thorax using anatomically guided sampling and parallel Monte Carlo computing. Biomed Opt Express, 2(9). pp. 2449-2460. 10.1364/BOE.2.002449. Retrieved from https://hdl.handle.net/10161/11254.
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Professor in Radiology
Dr. Cristian T. Badea is a Professor in the Department of Radiology and faculty in the Departments of Biomedical Engineering and Medical Physics. His research interests are in the physics and biomedical applications of computed tomography (CT), micro-CT, tomosynthesis, and image reconstruction algorithms.
Charles E. Putman University Distinguished Professor of Radiology
Dr. Johnson is the Charles E. Putman University Professor of Radiology, Professor of Physics, and Biomedical Engineering, and Director of the Duke Center for In Vivo Microscopy (CIVM). The CIVM is an NIH/NIBIB national Biomedical Technology Resource Center with a mission to develop novel technologies for preclinical imaging (basic sciences) and apply the technologies to critical biomedical questions. Dr. Johnson was one of the first researchers to bring Paul Lauterbur's vision of magnetic resona
Assistant Professor of Radiology
Xiaofeng “Steve” Zhang graduated from Tsinghua University (China) in Chemical Engineering (B.E. in 1997), and received his graduate degrees from University of Illinois at Urbana-Champaign in Electrical Engineering (M.S. in 2003 and Ph.D. in 2005). He studies the interaction of light with biological tissue, with which to noninvasively probe various biomedical phenomena in vivo, and the means to tomographically visualize such phenomena.
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