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Profiling of experience-regulated proteins in the songbird auditory forebrain using quantitative proteomics.

dc.contributor.author Alzate, O
dc.contributor.author Jarvis, Erich David
dc.contributor.author Osorio, C
dc.contributor.author Pinaud, R
dc.coverage.spatial France
dc.date.accessioned 2015-12-22T04:35:42Z
dc.date.issued 2008-03
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/18364021
dc.identifier EJN6102
dc.identifier.uri http://hdl.handle.net/10161/11262
dc.description.abstract Auditory and perceptual processing of songs are required for a number of behaviors in songbirds such as vocal learning, territorial defense, mate selection and individual recognition. These neural processes are accompanied by increased expression of a few transcription factors, particularly in the caudomedial nidopallium (NCM), an auditory forebrain area believed to play a key role in auditory learning and song discrimination. However, these molecular changes are presumably part of a larger, yet uncharacterized, protein regulatory network. In order to gain further insight into this network, we performed two-dimensional differential in-gel expression (2D-DIGE) experiments, extensive protein quantification analyses, and tandem mass spectrometry in the NCM of adult songbirds hearing novel songs. A subset of proteins was selected for immunocytochemistry in NCM sections to confirm the 2D-DIGE findings and to provide additional quantitative and anatomical information. Using these methodologies, we found that stimulation of freely behaving birds with conspecific songs did not significantly impact the NCM proteome 5 min after stimulus onset. However, following 1 and 3 h of stimulation, a significant number of proteins were consistently regulated in NCM. These proteins spanned a range of functional categories that included metabolic enzymes, cytoskeletal molecules, and proteins involved in neurotransmitter secretion and calcium binding. Our findings suggest that auditory processing of vocal communication signals in freely behaving songbirds triggers a cascade of protein regulatory events that are dynamically regulated through activity-dependent changes in calcium levels.
dc.language eng
dc.relation.ispartof Eur J Neurosci
dc.relation.isversionof 10.1111/j.1460-9568.2008.06102.x
dc.subject Acoustic Stimulation
dc.subject Animals
dc.subject Auditory Pathways
dc.subject Female
dc.subject Finches
dc.subject Gene Expression Profiling
dc.subject Learning
dc.subject Nerve Tissue Proteins
dc.subject Prosencephalon
dc.subject Proteomics
dc.subject Songbirds
dc.subject Vocalization, Animal
dc.title Profiling of experience-regulated proteins in the songbird auditory forebrain using quantitative proteomics.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/18364021
pubs.begin-page 1409
pubs.end-page 1422
pubs.issue 6
pubs.organisational-group Basic Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Institute for Brain Sciences
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Neurobiology
pubs.organisational-group School of Medicine
pubs.organisational-group University Institutes and Centers
pubs.publication-status Published
pubs.volume 27
dc.identifier.eissn 1460-9568


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