Genome-Wide Assessment of Outer Membrane Vesicle Production in Escherichia coli.
Abstract
The production of outer membrane vesicles by Gram-negative bacteria has been well
documented; however, the mechanism behind the biogenesis of these vesicles remains
unclear. Here a high-throughput experimental method and systems-scale analysis was
conducted to determine vesiculation values for the whole genome knockout library of
Escherichia coli mutant strains (Keio collection). The resultant dataset quantitatively
recapitulates previously observed phenotypes and implicates nearly 150 new genes in
the process of vesiculation. Gene functional and biochemical pathway analyses suggest
that mutations that truncate outer membrane structures such as lipopolysaccharide
and enterobacterial common antigen lead to hypervesiculation, whereas mutants in oxidative
stress response pathways result in lower levels. This study expands and refines the
current knowledge regarding the cellular pathways required for outer membrane vesiculation
in E. coli.
Type
Journal articleSubject
Bacterial Outer Membrane ProteinsCell Membrane
Cytoplasmic Vesicles
Escherichia coli
Genome, Bacterial
Mutation
Phenotype
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https://hdl.handle.net/10161/11279Published Version (Please cite this version)
10.1371/journal.pone.0139200Publication Info
Kulp, Adam J; Sun, Bo; Ai, Teresa; Manning, Andrew J; Orench-Rivera, Nichole; Schmid,
Amy K; & Kuehn, Meta J (2015). Genome-Wide Assessment of Outer Membrane Vesicle Production in Escherichia coli. PLoS One, 10(9). pp. e0139200. 10.1371/journal.pone.0139200. Retrieved from https://hdl.handle.net/10161/11279.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Margarethe Joanna Kuehn
Associate Professor of Biochemistry
Enterotoxigenic E. coli (ETEC) causes traveler's diarrhea and infant mortality in
underdeveloped countries, and Pseudomonas aeruginosa is an opportunistic pathogen
for immunocompromised patients. Like all gram negative bacteria studied to date, ETEC
and P. aeruginosa produce small outer membrane vesicles that can serve as delivery
"bombs" to host tissues. Vesicles contain a subset of outer membrane and soluble periplasmic
proteins and lipids. In tissues and sera of infected hosts, vesicles have
Amy K. Schmid
David M. Goodner Associate Professor
Research in my lab seeks to elucidate how cells make decisions in response to environmental
cues. My particular focus is on how networks of molecules interact within free-living
microbial cells. These networks govern the decision to grow when conditions are optimal
or deploy damage repair systems when faced with stress. I study microbial stress responses
in extremophiles of the domainArchaea, which represent extreme examples of microbes
surviving damage by multiple stressors. These organisms rem
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