Genome-Wide Assessment of Outer Membrane Vesicle Production in Escherichia coli.
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The production of outer membrane vesicles by Gram-negative bacteria has been well documented; however, the mechanism behind the biogenesis of these vesicles remains unclear. Here a high-throughput experimental method and systems-scale analysis was conducted to determine vesiculation values for the whole genome knockout library of Escherichia coli mutant strains (Keio collection). The resultant dataset quantitatively recapitulates previously observed phenotypes and implicates nearly 150 new genes in the process of vesiculation. Gene functional and biochemical pathway analyses suggest that mutations that truncate outer membrane structures such as lipopolysaccharide and enterobacterial common antigen lead to hypervesiculation, whereas mutants in oxidative stress response pathways result in lower levels. This study expands and refines the current knowledge regarding the cellular pathways required for outer membrane vesiculation in E. coli.
SubjectBacterial Outer Membrane Proteins
Published Version (Please cite this version)10.1371/journal.pone.0139200
Publication InfoAi, T; Kuehn, Margarethe Joanna; Kulp, AJ; Manning, AJ; Orench-Rivera, Nichole; Schmid, AK; & Sun, Bo (2015). Genome-Wide Assessment of Outer Membrane Vesicle Production in Escherichia coli. PLoS One, 10(9). pp. e0139200. 10.1371/journal.pone.0139200. Retrieved from http://hdl.handle.net/10161/11279.
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Associate Professor of Biochemistry
Enterotoxigenic E. coli (ETEC) causes traveler's diarrhea and infant mortality in underdeveloped countries, and Pseudomonas aeruginosa is an opportunistic pathogen for immunocompromised patients. Like all gram negative bacteria studied to date, ETEC and P. aeruginosa produce small outer membrane vesicles that can serve as delivery "bombs" to host tissues. Vesicles contain a subset of outer membrane and soluble periplasmic proteins and lipids. In tissues and sera of infected hosts,