Cell cycle Start is coupled to entry into the yeast metabolic cycle across diverse strains and growth rates.
Abstract
Cells have evolved oscillators with different frequencies to coordinate periodic processes.
Here we studied the interaction of two oscillators, the cell division cycle (CDC)
and the yeast metabolic cycle (YMC), in budding yeast. Previous work suggested that
the CDC and YMC interact to separate high oxygen consumption (HOC) from DNA replication
to prevent genetic damage. To test this hypothesis, we grew diverse strains in chemostat
and measured DNA replication and oxygen consumption with high temporal resolution
at different growth rates. Our data showed that HOC is not strictly separated from
DNA replication; rather, cell cycle Start is coupled with the initiation of HOC and
catabolism of storage carbohydrates. The logic of this YMC-CDC coupling may be to
ensure that DNA replication and cell division occur only when sufficient cellular
energy reserves have accumulated. Our results also uncovered a quantitative relationship
between CDC period and YMC period across different strains. More generally, our approach
shows how studies in genetically diverse strains efficiently identify robust phenotypes
and steer the experimentalist away from strain-specific idiosyncrasies.
Type
Journal articleSubject
Biological ClocksCell Cycle
Cell Division
DNA Replication
Oxygen
Oxygen Consumption
Partial Pressure
Saccharomycetales
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https://hdl.handle.net/10161/11291Published Version (Please cite this version)
10.1091/mbc.E15-07-0454Publication Info
Burnetti, Anthony J; Aydin, Mert; & Buchler, Nicolas E (2016). Cell cycle Start is coupled to entry into the yeast metabolic cycle across diverse
strains and growth rates. Mol Biol Cell, 27(1). pp. 64-74. 10.1091/mbc.E15-07-0454. Retrieved from https://hdl.handle.net/10161/11291.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Nicolas Buchler
Assistant Professor of Biology
Our lab is interested in the systems biology and evolution of epigenetic switches
(bistability) and clocks (oscillators) in gene regulatory networks, two functions
that are essential for patterning, cell proliferation, and differentiation in biological
systems. We also study biochemical oscillators such as the cell cycle, metabolic rhythms,
and circadian clocks, which co-exist in the same cells and interact with one another
through shared resources.

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