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Cell cycle Start is coupled to entry into the yeast metabolic cycle across diverse strains and growth rates. Burnetti, Anthony J Aydin, Mert Buchler, Nicolas E
dc.coverage.spatial United States 2015-12-09T16:32:57Z 2015-12-30T14:29:17Z 2016-01-01
dc.identifier mbc.E15-07-0454
dc.description.abstract Cells have evolved oscillators with different frequencies to coordinate periodic processes. Here we studied the interaction of two oscillators, the cell division cycle (CDC) and the yeast metabolic cycle (YMC), in budding yeast. Previous work suggested that the CDC and YMC interact to separate high oxygen consumption (HOC) from DNA replication to prevent genetic damage. To test this hypothesis, we grew diverse strains in chemostat and measured DNA replication and oxygen consumption with high temporal resolution at different growth rates. Our data showed that HOC is not strictly separated from DNA replication; rather, cell cycle Start is coupled with the initiation of HOC and catabolism of storage carbohydrates. The logic of this YMC-CDC coupling may be to ensure that DNA replication and cell division occur only when sufficient cellular energy reserves have accumulated. Our results also uncovered a quantitative relationship between CDC period and YMC period across different strains. More generally, our approach shows how studies in genetically diverse strains efficiently identify robust phenotypes and steer the experimentalist away from strain-specific idiosyncrasies.
dc.language eng
dc.publisher American Society for Cell Biology (ASCB)
dc.relation.ispartof Mol Biol Cell
dc.relation.isversionof 10.1091/mbc.E15-07-0454
dc.relation.replaces 10161/11135
dc.subject Biological Clocks
dc.subject Cell Cycle
dc.subject Cell Division
dc.subject DNA Replication
dc.subject Oxygen
dc.subject Oxygen Consumption
dc.subject Partial Pressure
dc.subject Saccharomycetales
dc.title Cell cycle Start is coupled to entry into the yeast metabolic cycle across diverse strains and growth rates.
dc.type Journal article Buchler, Nicolas E|0516712
pubs.begin-page 64
pubs.declined 2016-02-12T16:41:03.771-0500
pubs.declined 2016-02-18T16:47:52.200-0500
pubs.declined 2016-03-21T11:40:13.534-0400
pubs.declined 2016-05-02T11:40:37.305-0400
pubs.end-page 74
pubs.issue 1
pubs.organisational-group Biology
pubs.organisational-group Duke
pubs.organisational-group Physics
pubs.organisational-group Trinity College of Arts & Sciences
pubs.publication-status Published
pubs.volume 27
dc.identifier.eissn 1939-4586
duke.contributor.orcid Buchler, Nicolas E|0000-0003-3940-3432

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