ALERT: This system is being upgraded on Tuesday December 12. It will not be available for use for several hours that day while the upgrade is in progress. Deposits to DukeSpace will be disabled on Monday December 11, so no new items are to be added to the repository while the upgrade is in progress. Everything should be back to normal by the end of day, December 12.
Cell cycle Start is coupled to entry into the yeast metabolic cycle across diverse strains and growth rates.
|dc.contributor.author||Burnetti, Anthony J|
|dc.contributor.author||Buchler, Nicolas E|
|dc.description.abstract||Cells have evolved oscillators with different frequencies to coordinate periodic processes. Here we studied the interaction of two oscillators, the cell division cycle (CDC) and the yeast metabolic cycle (YMC), in budding yeast. Previous work suggested that the CDC and YMC interact to separate high oxygen consumption (HOC) from DNA replication to prevent genetic damage. To test this hypothesis, we grew diverse strains in chemostat and measured DNA replication and oxygen consumption with high temporal resolution at different growth rates. Our data showed that HOC is not strictly separated from DNA replication; rather, cell cycle Start is coupled with the initiation of HOC and catabolism of storage carbohydrates. The logic of this YMC-CDC coupling may be to ensure that DNA replication and cell division occur only when sufficient cellular energy reserves have accumulated. Our results also uncovered a quantitative relationship between CDC period and YMC period across different strains. More generally, our approach shows how studies in genetically diverse strains efficiently identify robust phenotypes and steer the experimentalist away from strain-specific idiosyncrasies.|
|dc.publisher||American Society for Cell Biology (ASCB)|
|dc.relation.ispartof||Mol Biol Cell|
|dc.title||Cell cycle Start is coupled to entry into the yeast metabolic cycle across diverse strains and growth rates.|
|duke.contributor.id||Buchler, Nicolas E|0516712|
|pubs.organisational-group||Trinity College of Arts & Sciences|
|duke.contributor.orcid||Buchler, Nicolas E|0000-0003-3940-3432|
Files in this item
This item appears in the following Collection(s)
- Scholarly Articles