In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. II. A common clonal origin for periarteriolar lymphoid sheath-associated foci and germinal centers.
Abstract
In the genetically restricted response that follows immunization with (4-hydroxy-3-nitrophenyl)acetyl
coupled to protein carriers, two distinct populations of B cells are observed in the
spleens of C57BL/6 mice. By 48 h postimmunization, foci of antigen-binding B cells
appear along the periphery of the periarteriolar lymphoid sheaths. These foci expand
to contain large numbers of antibody-forming cells that neither bind the lectin, peanut
agglutinin, nor mutate the rearranged immunoglobulin variable region loci. Germinal
centers containing peanut agglutinin-positive B cells can be observed by 96-120 h
after immunization. Although specific for the immunizing hapten, these B cells do
not produce substantial amounts of antibody, but are the population that undergoes
somatic hypermutation and affinity-driven selection. Both focus and germinal center
populations are pauciclonal, founded, on average, by three or fewer B lymphocytes.
Despite the highly specialized roles of the focus (early antibody production) and
germinal center (higher affinity memory cells) B cell populations, analysis of VH
to D to JH joins in neighboring foci and germinal centers demonstrate that these B
cell populations have a common clonal origin.
Type
Journal articleSubject
AnimalsB-Lymphocytes
Base Sequence
Clone Cells
DNA
Female
Haptens
Immunoenzyme Techniques
Immunoglobulin Heavy Chains
Lymphoid Tissue
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Nitrophenols
Phenylacetates
Sequence Homology, Nucleic Acid
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Show full item recordScholars@Duke
Garnett H. Kelsoe
James B. Duke Distinguished Professor of Immunology
1. Lymphocyte development and antigen-driven diversification of immunoglobulin and
T cell antigen receptor genes. 2. The germinal center reaction and mechanisms for
clonal selection and self - tolerance. The origins of autoimmunity. 3. Interaction
of innate- and adaptive immunity and the role of inflammation in lymphoid organogenesis.
4. The role of secondary V(D)J gene rearrangment in lymphocyte development and malignancies.
5. Mathematical modeling of immune responses,

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