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In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. II. A common clonal origin for periarteriolar lymphoid sheath-associated foci and germinal centers. Jacob, J Kelsoe, G
dc.coverage.spatial United States 2016-01-08T18:08:20Z 1992-09-01
dc.identifier.issn 0022-1007
dc.description.abstract In the genetically restricted response that follows immunization with (4-hydroxy-3-nitrophenyl)acetyl coupled to protein carriers, two distinct populations of B cells are observed in the spleens of C57BL/6 mice. By 48 h postimmunization, foci of antigen-binding B cells appear along the periphery of the periarteriolar lymphoid sheaths. These foci expand to contain large numbers of antibody-forming cells that neither bind the lectin, peanut agglutinin, nor mutate the rearranged immunoglobulin variable region loci. Germinal centers containing peanut agglutinin-positive B cells can be observed by 96-120 h after immunization. Although specific for the immunizing hapten, these B cells do not produce substantial amounts of antibody, but are the population that undergoes somatic hypermutation and affinity-driven selection. Both focus and germinal center populations are pauciclonal, founded, on average, by three or fewer B lymphocytes. Despite the highly specialized roles of the focus (early antibody production) and germinal center (higher affinity memory cells) B cell populations, analysis of VH to D to JH joins in neighboring foci and germinal centers demonstrate that these B cell populations have a common clonal origin.
dc.language eng
dc.publisher Rockefeller University Press
dc.relation.ispartof J Exp Med
dc.subject Animals
dc.subject B-Lymphocytes
dc.subject Base Sequence
dc.subject Clone Cells
dc.subject DNA
dc.subject Female
dc.subject Haptens
dc.subject Immunoenzyme Techniques
dc.subject Immunoglobulin Heavy Chains
dc.subject Lymphoid Tissue
dc.subject Mice
dc.subject Mice, Inbred C57BL
dc.subject Molecular Sequence Data
dc.subject Nitrophenols
dc.subject Phenylacetates
dc.subject Sequence Homology, Nucleic Acid
dc.title In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. II. A common clonal origin for periarteriolar lymphoid sheath-associated foci and germinal centers.
dc.type Journal article Kelsoe, G|0205291
pubs.begin-page 679
pubs.end-page 687
pubs.issue 3
pubs.organisational-group Basic Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Duke Human Vaccine Institute
pubs.organisational-group Immunology
pubs.organisational-group Institutes and Centers
pubs.organisational-group School of Medicine
pubs.publication-status Published
pubs.volume 176

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