NgBR is essential for endothelial cell glycosylation and vascular development.
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NgBR is a transmembrane protein identified as a Nogo-B-interacting protein and recently has been shown to be a subunit required for cis-prenyltransferase (cisPTase) activity. To investigate the integrated role of NgBR in vascular development, we have characterized endothelial-specific NgBR knockout embryos. Here, we show that endothelial-specific NgBR knockout results in embryonic lethality due to vascular development defects in yolk sac and embryo proper. Loss of NgBR in endothelial cells reduces proliferation and promotes apoptosis of the cells largely through defects in the glycosylation of key endothelial proteins including VEGFR2, VE-cadherin, and CD31, and defective glycosylation can be rescued by treatment with the end product of cisPTase activity, dolichol phosphate. Moreover, NgBR functions in endothelial cells during embryogenesis are Nogo-B independent. These data uniquely show the importance of NgBR and protein glycosylation during vascular development.
Protein Processing, Post-Translational
Receptors, Cell Surface
Vascular Endothelial Growth Factor Receptor-2
Published Version (Please cite this version)10.15252/embr.201540789
Publication InfoGrabińska, KA; Guan, Ziqiang; Park, EJ; & Sessa, WC (2016). NgBR is essential for endothelial cell glycosylation and vascular development. EMBO Rep, 17(2). pp. 167-177. 10.15252/embr.201540789. Retrieved from https://hdl.handle.net/10161/11565.
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Research Professor in Biochemistry
We develop and apply mass spectrometry techniques to address biochemical and biomedical questions that are lipid-related. Research projects include: 1) Structural lipidomics o Develop and apply high resolution tandem mass spectrometry-based lipidomics for the discovery, structural elucidation and functional study of novel lipids. 2) Elucidation of novel pathways/enzymes of lipid biosynthesis and metabolism o Genetic, biochemical and MS a