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TRPV channel-mediated calcium transients in nociceptor neurons are dispensable for avoidance behaviour.

dc.contributor.author Anishkin, A
dc.contributor.author Chintapalli, SV
dc.contributor.author Kanju, Patrick
dc.contributor.author Liedtke, Wolfgang Bernhard
dc.contributor.author Lindy, AS
dc.contributor.author Parekh, Puja K
dc.contributor.author Patterson, RL
dc.contributor.author Tsvilovskyy, V
dc.contributor.author van Rossum, DB
dc.contributor.author Zhu, R
dc.coverage.spatial England
dc.date.accessioned 2016-03-01T14:18:23Z
dc.date.issued 2014-09-02
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/25178952
dc.identifier ncomms5734
dc.identifier.uri https://hdl.handle.net/10161/11664
dc.description.abstract Animals need to sense and react to potentially dangerous environments. TRP ion channels participate in nociception, presumably via Ca(2+) influx, in most animal species. However, the relationship between ion permeation and animals' nocifensive behaviour is unknown. Here we use an invertebrate animal model with relevance for mammalian pain. We analyse the putative selectivity filter of OSM-9, a TRPV channel, in osmotic avoidance behaviour of Caenorhabditis elegans. Using mutagenized OSM-9 expressed in the head nociceptor neuron, ASH, we study nocifensive behaviour and Ca(2+) influx. Within the selectivity filter, M(601)-F(609), Y604G strongly reduces avoidance behaviour and eliminates Ca(2+) transients. Y604F also abolishes Ca(2+) transients in ASH, while sustaining avoidance behaviour, yet it disrupts behavioral plasticity. Homology modelling of the OSM-9 pore suggests that Y(604) may assume a scaffolding role. Thus, aromatic residues in the OSM-9 selectivity filter are critical for pain behaviour and ion permeation. These findings have relevance for understanding evolutionary roots of mammalian nociception.
dc.language eng
dc.relation.ispartof Nat Commun
dc.relation.isversionof 10.1038/ncomms5734
dc.subject Amino Acid Sequence
dc.subject Amino Acid Substitution
dc.subject Animals
dc.subject Avoidance Learning
dc.subject Caenorhabditis elegans
dc.subject Caenorhabditis elegans Proteins
dc.subject Calcium
dc.subject Calcium Signaling
dc.subject Gene Expression
dc.subject Ion Transport
dc.subject Models, Molecular
dc.subject Molecular Sequence Data
dc.subject Mutation
dc.subject Nerve Tissue Proteins
dc.subject Nociception
dc.subject Nociceptors
dc.subject Recombinant Fusion Proteins
dc.subject Sequence Alignment
dc.subject Structural Homology, Protein
dc.subject TRPV Cation Channels
dc.title TRPV channel-mediated calcium transients in nociceptor neurons are dispensable for avoidance behaviour.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/25178952
pubs.begin-page 4734
pubs.organisational-group Anesthesiology
pubs.organisational-group Basic Science Departments
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Institute for Brain Sciences
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Neurobiology
pubs.organisational-group Neurology
pubs.organisational-group Neurology, Headache and Pain
pubs.organisational-group School of Medicine
pubs.organisational-group University Institutes and Centers
pubs.publication-status Published online
pubs.volume 5
dc.identifier.eissn 2041-1723


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