Direct and cost-efficient hyperpolarization of long-lived nuclear spin states on universal (15)N2-diazirine molecular tags.
Abstract
Conventional magnetic resonance (MR) faces serious sensitivity limitations which can
be overcome by hyperpolarization methods, but the most common method (dynamic nuclear
polarization) is complex and expensive, and applications are limited by short spin
lifetimes (typically seconds) of biologically relevant molecules. We use a recently
developed method, SABRE-SHEATH, to directly hyperpolarize (15)N2 magnetization and
long-lived (15)N2 singlet spin order, with signal decay time constants of 5.8 and
23 minutes, respectively. We find >10,000-fold enhancements generating detectable
nuclear MR signals that last for over an hour. (15)N2-diazirines represent a class
of particularly promising and versatile molecular tags, and can be incorporated into
a wide range of biomolecules without significantly altering molecular function.
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Journal articlePermalink
https://hdl.handle.net/10161/11770Published Version (Please cite this version)
10.1126/sciadv.1501438Publication Info
(2016). Direct and cost-efficient hyperpolarization of long-lived nuclear spin states on universal
(15)N2-diazirine molecular tags. Sci Adv, 2(3). pp. e1501438. 10.1126/sciadv.1501438. Retrieved from https://hdl.handle.net/10161/11770.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Volker Blum
Associate Professor in the Department of Mechanical Engineering and Materials Science
Volker Blum heads the "Ab initio materials simulations" group at Duke University.
Dr. Blum's research focuses on first-principles computational materials science: using
the fundamental laws of quantum mechanics to predict the properties of real materials
from the atomic scale on upwards. Specific focus areas are interface and nanoscale
systems with electronic and energy applications, as well as work on molecular structure
and spectroscopy. He
William Huhn
Postdoctoral Associate
Computational materials scientist specializing in high performance resources and next-generation
hardware architectures.
Steven Malcolmson
Associate Professor of Chemistry
The discovery of catalysts is of great importance to the practice of modern synthetic
chemistry, both to improve upon the existing catalog of chemical transformations and
to generate new modes of reactivity. Research in the Malcolmson lab focuses on the
discovery of novel methods for the efficient and selective synthesis of small molecule
scaffolds through the design and development of new catalysts. In these transformations,
we seek to
Thomas Theis
Assistant Research Professor of Chemistry
Theis' research is at the intersection of Spin Physics and Hyperpolarization Chemistry.
It has applications in the study of biochemical dynamics and molecular imaging. The
Theis lab drives innovation of magnetic resonance tools and techniques to break the
sensitivity limits of NMR and MRI. The innovations enable i) biochemical structure
elucidation with unprecedented limits of detection, and ii) molecular imaging to spy
on mole
Qiu Wang
Professor of Chemistry
Research in the Wang group aims to answer fundamental questions that lie at the interface
of chemistry and biology. In particular, we are interested in developing small-molecule
based probes and methods to understand the cause of disease with an emphasis on identifying
potential therapeutic agents towards cancer and neurodegenerative disorders.
Bioactive molecules as probes in human biology and disease. Starting from naturally
occurring molecules that possess u
Warren S. Warren
James B. Duke Distinguished Professor of Chemistry
Our work focuses on the design and application of what might best be called novel
pulsed techniques, using controlled radiation fields to alter dynamics. The heart
of the work is chemical physics, and most of what we do is ultrafast laser spectroscopy
or nuclear magnetic resonance. It generally involves an intimate mixture of theory
and experiment: recent publications are roughly an equal mix of pencil- and-paper
theory, computer calculations with our workstations, and experiments. Collabo
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