Direct and cost-efficient hyperpolarization of long-lived nuclear spin states on universal (15)N2-diazirine molecular tags.
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Conventional magnetic resonance (MR) faces serious sensitivity limitations which can be overcome by hyperpolarization methods, but the most common method (dynamic nuclear polarization) is complex and expensive, and applications are limited by short spin lifetimes (typically seconds) of biologically relevant molecules. We use a recently developed method, SABRE-SHEATH, to directly hyperpolarize (15)N2 magnetization and long-lived (15)N2 singlet spin order, with signal decay time constants of 5.8 and 23 minutes, respectively. We find >10,000-fold enhancements generating detectable nuclear MR signals that last for over an hour. (15)N2-diazirines represent a class of particularly promising and versatile molecular tags, and can be incorporated into a wide range of biomolecules without significantly altering molecular function.
Published Version (Please cite this version)10.1126/sciadv.1501438
Publication InfoBlum, Volker; Chekmenev, EY; Claytor, KE; Feng, Y; Huhn, WP; Logan, AW; ... Warren, WS (2016). Direct and cost-efficient hyperpolarization of long-lived nuclear spin states on universal (15)N2-diazirine molecular tags. Sci Adv, 2(3). pp. e1501438. 10.1126/sciadv.1501438. Retrieved from https://hdl.handle.net/10161/11770.
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Associate Professor in the Department of Mechanical Engineering and Materials Science
Volker Blum heads the "Ab initio materials simulations" group at Duke University. Dr. Blum's research focuses on "first-principles" computational materials science: using the fundamental laws of quantum mechanics to predict the properties of real materials from the atomic scale on upwards. Specific focus areas are interface and nanoscale systems with electronic and energy applications, as well as work on molecular structure and spectroscopy. R
Assistant Research Professor of Chemistry
Theis' research is at the intersection of Spin Physics and Hyperpolarization Chemistry. It has applications in the study of biochemical dynamics and molecular imaging. The Theis lab drives innovation of magnetic resonance tools and techniques to break the sensitivity limits of NMR and MRI. The innovations enable i) biochemical structure elucidation with unprecedented limits of detection, and ii) molecular imaging to spy on mole
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