A Therapeutic Antibody for Cancer, Derived from Single Human B Cells.
Repository Usage Stats
Some patients with cancer never develop metastasis, and their host response might provide cues for innovative treatment strategies. We previously reported an association between autoantibodies against complement factor H (CFH) and early-stage lung cancer. CFH prevents complement-mediated cytotoxicity (CDC) by inhibiting formation of cell-lytic membrane attack complexes on self-surfaces. In an effort to translate these findings into a biologic therapy for cancer, we isolated and expressed DNA sequences encoding high-affinity human CFH antibodies directly from single, sorted B cells obtained from patients with the antibody. The co-crystal structure of a CFH antibody-target complex shows a conformational change in the target relative to the native structure. This recombinant CFH antibody causes complement activation and release of anaphylatoxins, promotes CDC of tumor cell lines, and inhibits tumor growth in vivo. The isolation of anti-tumor antibodies derived from single human B cells represents an alternative paradigm in antibody drug discovery.
Published Version (Please cite this version)10.1016/j.celrep.2016.04.038
Publication InfoBushey, Ryan T; Moody, M Anthony; Nicely, Nathan L; Haynes, Barton F; Alam, S Munir; Keir, Stephen T; ... Patz, Edward F (2016). A Therapeutic Antibody for Cancer, Derived from Single Human B Cells. Cell Rep, 15(7). pp. 1505-1513. 10.1016/j.celrep.2016.04.038. Retrieved from https://hdl.handle.net/10161/12221.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
More InfoShow full item record
Professor in Medicine
Research Interests. The Alam laboratory’s primary research is focused on understanding the biophysical properties of antigen-antibody binding and the molecular events of early B cell activation using the HIV-1 broadly neutralizing antibody (bnAb) lineage models. We are studying how HIV-1 Envelope proteins of varying affinities are sensed by B cells expressing HIV-1 bnAbs or their germline antigen receptors and initiate early signaling events for their activation. In the lon
Professor of Pathology
Outcome-based research on pathology of endometrial carcinoma, including prognostic significance of histologic features of endometrial carcinoma, variants of endometrial carcinoma, definitions of atypia and well-differentiated carcinoma, and collaborative studies of oncogenes and tumor suppressor genes in endometrial carcinoma. Endometrial pathology, especially as it relates to molecular/genetic alterations in neoplasms. Ovarian pathology, especially as it relates to
Associate Professor in Radiology
There is longstanding evidence that invasive lung cancer is the end result of a multi-step process in which progressive molecular changes herald and accompany cytomorphologic changes. Our knowledge of these molecular events and the specific markers associated with the evolution from initiation to invasion is only partial. A number of specific biomarkers involved in oncogene activation or inactivation of tumor suppressor genes have been identified, but no single marker to date has been shown to h
Assistant Professor in Radiology
Frederic M. Hanes Distinguished Professor of Medicine
The Haynes lab is studying host innate and adaptive immune responses to the human immunodeficiency virus (HIV), tuberculosis (TB), and influenza in order to find the enabling technology to make preventive vaccines against these three major infectious diseases. Mucosal Immune Responses in Acute HIV Infection The Haynes lab is working to determine why broadly neutralizing antibodies are rarely made in acute HIV infection (AHI), currently a major obstacle in the de
Professor in Neurosurgery
Brain Tumors, Preclinical Testing, Translational Research
Adjunct Professor in the Department of Medicine
Dr. Liao is a Professor of Medicine and Research Director of Duke Human Vaccine Institute. Dr. Liao is a MD virologistt rained in China. In early 1980’s, Dr. Liao made major contributions to the first isolation of epidemic hemorrhagic fever virus (hataanvirus) from Apodemus agraius using tissue culture in China. The successful identification and isolation of Hataanvirus enabled the early diagnosis and treatment of the disease, and advancement of HFRS research towards prevention by de
Associate Professor of Pediatrics
Tony Moody, MD is an Associate Professor in the Department of Pediatrics, Division of Infectious Diseases and the Department of Immunology at Duke University Medical Center. Research in the Moody lab is focused on understanding the B cell responses during infection, vaccination, and disease. The lab has become a resource for human phenotyping, flow characterization, staining and analysis at the Duke Human Vaccine Institute (DHVI). The Moody lab is currently funded to study influenza, syphilis
Assistant Professor in Medicine
Nathan Nicely, Ph.D., is an Assistant Professor of Medicine with the Duke Human Vaccine Institute and Director of the Duke University X-ray Crystallography shared resource. His major research interests lie in the structural biology of anti-HIV antibodies and HIV virion coat proteins. Dr. Nicely received his Ph.D. in Structural and Molecular Biochemistry from NC State University in 2005 with Dr. Carla Mattos. He then completed a postdoctoral fellowship with Dr. Al Claiborne in the Center for S
James and Alice Chen Distinguished Professor of Radiology
There are numerous ongoing clinical studies primarily focused on the early detection of cancer. The basic science investigations in our laboratory concentration on three fundamental translational areas, 1) Development of molecular imaging probes - We have used several different approaches to develop novel imaging probes that characterize and phenotype tumors. 2) Discovery of novel lung cancer biomarkers - We ex
Adjunct Associate Professor in the Department of Biostatistics and Bioinformatics
Statistical modelling, data analysis. Analysis of multiple observer studies. Analysis of complex data. Modelling tumor growth. Translation in drug discovery.Statistical analysis of images: cellular and medical imaging. Statistics of shape, structure and spatial arrangement.
Alphabetical list of authors with Scholars@Duke profiles.