Parallel on-axis holographic phase microscopy of biological cells and unicellular microorganism dynamics.
Abstract
We apply a wide-field quantitative phase microscopy technique based on parallel two-step
phase-shifting on-axis interferometry to visualize live biological cells and microorganism
dynamics. The parallel on-axis holographic approach is more efficient with camera
spatial bandwidth consumption compared to previous off-axis approaches and thus can
capture finer sample spatial details, given a limited spatial bandwidth of a specific
digital camera. Additionally, due to the parallel acquisition mechanism, the approach
is suitable for visualizing rapid dynamic processes, permitting an interferometric
acquisition rate equal to the camera frame rate. The method is demonstrated experimentally
through phase microscopy of neurons and unicellular microorganisms.
Type
Journal articleSubject
AnimalsCells, Cultured
Computer-Aided Design
Equipment Design
Equipment Failure Analysis
Euglena gracilis
Holography
Microscopy, Phase-Contrast
Neurons
Rats
Reproducibility of Results
Sensitivity and Specificity
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Show full item recordScholars@Duke
Thomas Mark Newpher
Assistant Professor of the Practice of Psychology and Neuroscience
Dr. Newpher teaches and advises for Duke's Undergraduate Studies in Neuroscience program. He
also directs the Summer Neuroscience Program of Research in the Duke Institute for
Brain Sciences. Dr. Newpher earned his Ph.D. in molecular biology from Case Western
Reserve University. He then came to Duke to receive postdoctoral training in the Department
of Neurobiology, where his research focused on identifying key molecular mechanisms
that underlie learning-related synaptic
Adam P. Wax
Professor of Biomedical Engineering
Dr. Wax's research interests include optical spectroscopy for early cancer detection,
novel microscopy and
interferometry techniques.
The study of intact, living cells with optical spectroscopy offers the opportunity
to observe cellular structure, organization and dynamics in a way that is not possible
with traditional methods. We have developed a set of novel spectroscopic techniques
for measuring spatial, temporal and refractive structure on sub-hertz and sub-wavelength
scales based
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