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Parallel on-axis holographic phase microscopy of biological cells and unicellular microorganism dynamics.

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Date
2010-05-20
Authors
Ehlers, MD
Newpher, Thomas Mark
Shaked, NT
Wax, A
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Abstract
We apply a wide-field quantitative phase microscopy technique based on parallel two-step phase-shifting on-axis interferometry to visualize live biological cells and microorganism dynamics. The parallel on-axis holographic approach is more efficient with camera spatial bandwidth consumption compared to previous off-axis approaches and thus can capture finer sample spatial details, given a limited spatial bandwidth of a specific digital camera. Additionally, due to the parallel acquisition mechanism, the approach is suitable for visualizing rapid dynamic processes, permitting an interferometric acquisition rate equal to the camera frame rate. The method is demonstrated experimentally through phase microscopy of neurons and unicellular microorganisms.
Type
Journal article
Subject
Animals
Cells, Cultured
Computer-Aided Design
Equipment Design
Equipment Failure Analysis
Euglena gracilis
Holography
Microscopy, Phase-Contrast
Neurons
Rats
Reproducibility of Results
Sensitivity and Specificity
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https://hdl.handle.net/10161/12464
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Scholars@Duke

Wax

Adam P. Wax

Professor of Biomedical Engineering
Dr. Wax's research interests include optical spectroscopy for early cancer detection, novel microscopy and interferometry techniques. The study of intact, living cells with optical spectroscopy offers the opportunity to observe cellular structure, organization and dynamics in a way that is not possible with traditional methods. We have developed a set of novel spectroscopic techniques for measuring spatial, temporal and refractive structure on sub-hertz and sub-wavelength scales based
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