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Parallel on-axis holographic phase microscopy of biological cells and unicellular microorganism dynamics.

dc.contributor.author Ehlers, MD
dc.contributor.author Newpher, Thomas Mark
dc.contributor.author Shaked, NT
dc.contributor.author Wax, A
dc.coverage.spatial United States
dc.date.accessioned 2011-06-21T17:27:39Z
dc.date.accessioned 2016-07-14T19:39:27Z
dc.date.issued 2010-05-20
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/20490249
dc.identifier 199509
dc.identifier.uri https://hdl.handle.net/10161/12464
dc.description.abstract We apply a wide-field quantitative phase microscopy technique based on parallel two-step phase-shifting on-axis interferometry to visualize live biological cells and microorganism dynamics. The parallel on-axis holographic approach is more efficient with camera spatial bandwidth consumption compared to previous off-axis approaches and thus can capture finer sample spatial details, given a limited spatial bandwidth of a specific digital camera. Additionally, due to the parallel acquisition mechanism, the approach is suitable for visualizing rapid dynamic processes, permitting an interferometric acquisition rate equal to the camera frame rate. The method is demonstrated experimentally through phase microscopy of neurons and unicellular microorganisms.
dc.language eng
dc.language.iso en_US
dc.relation.ispartof Appl Opt
dc.relation.replaces http://hdl.handle.net/10161/4206
dc.relation.replaces 10161/4206
dc.subject Animals
dc.subject Cells, Cultured
dc.subject Computer-Aided Design
dc.subject Equipment Design
dc.subject Equipment Failure Analysis
dc.subject Euglena gracilis
dc.subject Holography
dc.subject Microscopy, Phase-Contrast
dc.subject Neurons
dc.subject Rats
dc.subject Reproducibility of Results
dc.subject Sensitivity and Specificity
dc.title Parallel on-axis holographic phase microscopy of biological cells and unicellular microorganism dynamics.
dc.title.alternative
dc.type Journal article
dc.description.version Version of Record
duke.date.pubdate 2010-5-20
duke.description.issue 15
duke.description.volume 49
dc.relation.journal Applied Optics
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/20490249
pubs.begin-page 2872
pubs.end-page 2878
pubs.issue 15
pubs.organisational-group Biomedical Engineering
pubs.organisational-group Duke
pubs.organisational-group Duke Institute for Brain Sciences
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Pratt School of Engineering
pubs.organisational-group University Institutes and Centers
pubs.publication-status Published
pubs.volume 49
dc.identifier.eissn 1539-4522


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