Role of Scd5, a protein phosphatase-1 targeting protein, in phosphoregulation of Sla1 during endocytosis.
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Phosphorylation regulates assembly and disassembly of proteins during endocytosis. In yeast, Prk1 and Ark1 phosphorylate factors after vesicle internalization leading to coat disassembly. Scd5, a protein phosphatase-1 (PP1)-targeting subunit, is proposed to regulate dephosphorylation of Prk1/Ark1 substrates to promote new rounds of endocytosis. In this study we analyzed scd5-PP1Δ2, a mutation causing impaired PP1 binding. scd5-PP1Δ2 caused hyperphosphorylation of several Prk1 endocytic targets. Live-cell imaging of 15 endocytic components in scd5-PP1Δ2 revealed that most factors arriving before the invagination/actin phase of endocytosis had delayed lifetimes. Severely affected were early factors and Sla2 (Hip1R homolog), whose lifetime was extended nearly fourfold. In contrast, the lifetime of Sla1, a Prk1 target, was extended less than twofold, but its cortical recruitment was significantly reduced. Delayed Sla2 dynamics caused by scd5-PP1Δ2 were suppressed by SLA1 overexpression. This was dependent on the LxxQxTG repeats (SR) of Sla1, which are phosphorylated by Prk1 and bind Pan1, another Prk1 target, in the dephosphorylated state. Without the SR, Sla1ΔSR was still recruited to the cell surface, but was less concentrated in cortical patches than Pan1. sla1ΔSR severely impaired endocytic progression, but this was partially suppressed by overexpression of LAS17, suggesting that without the SR region the SH3 region of Sla1 causes constitutive negative regulation of Las17 (WASp). These results demonstrate that Scd5/PP1 is important for recycling Prk1 targets to initiate new rounds of endocytosis and provide new mechanistic information on the role of the Sla1 SR domain in regulating progression to the invagination/actin phase of endocytosis.
Gene Expression Regulation, Fungal
Protein Phosphatase 1
Protein Structure, Tertiary
Saccharomyces cerevisiae Proteins
Wiskott-Aldrich Syndrome Protein
Published Version (Please cite this version)10.1242/jcs.098871
Publication InfoChang, JS; Chi, RJ; Lansley, T; Lemmon, Sandra K; Newpher, Thomas Mark; Segarra, VA; & Torres, OT (2012). Role of Scd5, a protein phosphatase-1 targeting protein, in phosphoregulation of Sla1 during endocytosis. J Cell Sci, 125(Pt 20). pp. 4728-4739. 10.1242/jcs.098871. Retrieved from http://hdl.handle.net/10161/12466.
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Assistant Professor of the Practice of Psychology and Neuroscience
Dr. Newpher teaches and advises for Duke's Undergraduate Studies in Neuroscience program. He also directs the Summer Neuroscience Program of Research in the Duke Institute for Brain Sciences. Dr. Newpher earned his Ph.D. in molecular biology from Case Western Reserve University. He then came to Duke to receive postdoctoral training in the Department of Neurobiology, where his research focused on identifying key molecular mechanisms that underlie learning-related synaptic
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