Show simple item record

Transcriptomic Analysis of the Host Response and Innate Resilience to Enterotoxigenic Escherichia coli Infection in Humans.

dc.contributor.author Burke, T
dc.contributor.author Chakraborty, S
dc.contributor.author Ginsburg, Geoffrey Steven
dc.contributor.author Harro, CD
dc.contributor.author McClain, MT
dc.contributor.author Nicholson, BP
dc.contributor.author Sack, DA
dc.contributor.author Suchindran, Sunil
dc.contributor.author Tsalik, Ephraim L
dc.contributor.author Woods, Christopher Wildrick
dc.contributor.author Yang, WE
dc.coverage.spatial United States
dc.date.accessioned 2016-08-01T14:23:35Z
dc.date.issued 2016-05-01
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/26787651
dc.identifier jiv593
dc.identifier.uri https://hdl.handle.net/10161/12537
dc.description.abstract BACKGROUND: Enterotoxigenic Escherichia coli (ETEC) is a globally prevalent cause of diarrhea. Though usually self-limited, it can be severe and debilitating. Little is known about the host transcriptional response to infection. We report the first gene expression analysis of the human host response to experimental challenge with ETEC. METHODS: We challenged 30 healthy adults with an unattenuated ETEC strain, and collected serial blood samples shortly after inoculation and daily for 8 days. We performed gene expression analysis on whole peripheral blood RNA samples from subjects in whom severe symptoms developed (n = 6) and a subset of those who remained asymptomatic (n = 6) despite shedding. RESULTS: Compared with baseline, symptomatic subjects demonstrated significantly different expression of 406 genes highlighting increased immune response and decreased protein synthesis. Compared with asymptomatic subjects, symptomatic subjects differentially expressed 254 genes primarily associated with immune response. This comparison also revealed 29 genes differentially expressed between groups at baseline, suggesting innate resilience to infection. Drug repositioning analysis identified several drug classes with potential utility in augmenting immune response or mitigating symptoms. CONCLUSIONS: There are statistically significant and biologically plausible differences in host gene expression induced by ETEC infection. Differential baseline expression of some genes may indicate resilience to infection.
dc.language eng
dc.relation.ispartof J Infect Dis
dc.relation.isversionof 10.1093/infdis/jiv593
dc.subject E. coli
dc.subject bacterial infections
dc.subject diarrheal illness
dc.subject gene expression
dc.subject immune response
dc.subject microarrays
dc.subject Adult
dc.subject Enterotoxigenic Escherichia coli
dc.subject Escherichia coli Infections
dc.subject Female
dc.subject Gene Expression Profiling
dc.subject Host-Pathogen Interactions
dc.subject Humans
dc.subject Immunity, Innate
dc.subject Male
dc.subject Middle Aged
dc.subject Oligonucleotide Array Sequence Analysis
dc.subject RNA
dc.subject Transcriptome
dc.subject Young Adult
dc.title Transcriptomic Analysis of the Host Response and Innate Resilience to Enterotoxigenic Escherichia coli Infection in Humans.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/26787651
pubs.begin-page 1495
pubs.end-page 1504
pubs.issue 9
pubs.organisational-group Biomedical Engineering
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Global Health Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Cardiology
pubs.organisational-group Medicine, Infectious Diseases
pubs.organisational-group Pathology
pubs.organisational-group Pratt School of Engineering
pubs.organisational-group School of Medicine
pubs.organisational-group School of Nursing
pubs.organisational-group School of Nursing - Secondary Group
pubs.organisational-group University Institutes and Centers
pubs.publication-status Published
pubs.volume 213
dc.identifier.eissn 1537-6613


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record