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Modulation of murine breast tumor vascularity, hypoxia and chemotherapeutic response by exercise.

dc.contributor.author Betof, AS
dc.contributor.author Devi, GR
dc.contributor.author Dewhirst, Mark Wesley
dc.contributor.author Jones, LW
dc.contributor.author Landon, C
dc.contributor.author Lascola, Christopher David
dc.contributor.author Palmer, Gregory M
dc.contributor.author Scarbrough, Peter
dc.contributor.author Weitzel, D
dc.coverage.spatial United States
dc.date.accessioned 2016-08-02T17:37:41Z
dc.date.issued 2015-05
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/25780062
dc.identifier djv040
dc.identifier.uri http://hdl.handle.net/10161/12580
dc.description.abstract Exercise has been shown to improve postischemia perfusion of normal tissues; we investigated whether these effects extend to solid tumors. Estrogen receptor-negative (ER-, 4T1) and ER+ (E0771) tumor cells were implanted orthotopically into syngeneic mice (BALB/c, N = 11-12 per group) randomly assigned to exercise or sedentary control. Tumor growth, perfusion, hypoxia, and components of the angiogenic and apoptotic cascades were assessed by MRI, immunohistochemistry, western blotting, and quantitative polymerase chain reaction and analyzed with one-way and repeated measures analysis of variance and linear regression. All statistical tests were two-sided. Exercise statistically significantly reduced tumor growth and was associated with a 1.4-fold increase in apoptosis (sedentary vs exercise: 1544 cells/mm(2), 95% CI = 1223 to 1865 vs 2168 cells/mm(2), 95% CI = 1620 to 2717; P = .048), increased microvessel density (P = .004), vessel maturity (P = .006) and perfusion, and reduced intratumoral hypoxia (P = .012), compared with sedentary controls. We also tested whether exercise could improve chemotherapy (cyclophosphamide) efficacy. Exercise plus chemotherapy prolonged growth delay compared with chemotherapy alone (P < .001) in the orthotopic 4T1 model (n = 17 per group). Exercise is a potential novel adjuvant treatment of breast cancer.
dc.language eng
dc.relation.ispartof J Natl Cancer Inst
dc.relation.isversionof 10.1093/jnci/djv040
dc.subject Analysis of Variance
dc.subject Animals
dc.subject Antineoplastic Agents, Alkylating
dc.subject Apoptosis
dc.subject Cell Hypoxia
dc.subject Cell Line, Tumor
dc.subject Cell Proliferation
dc.subject Cyclophosphamide
dc.subject Exercise
dc.subject Female
dc.subject Humans
dc.subject Linear Models
dc.subject Mammary Neoplasms, Experimental
dc.subject Mice
dc.subject Mice, Inbred BALB C
dc.subject Microcirculation
dc.subject Neoplasm Transplantation
dc.subject Neovascularization, Pathologic
dc.subject Random Allocation
dc.subject Receptors, Estrogen
dc.subject Treatment Outcome
dc.title Modulation of murine breast tumor vascularity, hypoxia and chemotherapeutic response by exercise.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/25780062
pubs.issue 5
pubs.organisational-group Basic Science Departments
pubs.organisational-group Biomedical Engineering
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Duke-UNC Center for Brain Imaging and Analysis
pubs.organisational-group Institutes and Centers
pubs.organisational-group Neurobiology
pubs.organisational-group Pathology
pubs.organisational-group Pratt School of Engineering
pubs.organisational-group Radiation Oncology
pubs.organisational-group Radiology
pubs.organisational-group Radiology, Neuroradiology
pubs.organisational-group School of Medicine
pubs.organisational-group Surgery
pubs.organisational-group Surgery, Surgical Sciences
pubs.publication-status Published online
pubs.volume 107
dc.identifier.eissn 1460-2105


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