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Direct In Vivo Manipulation and Imaging of Calcium Transients in Neutrophils Identify a Critical Role for Leading-Edge Calcium Flux.

dc.contributor.author Tobin, DM
dc.coverage.spatial United States
dc.date.accessioned 2016-08-10T17:51:20Z
dc.date.issued 2015-12-15
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/26673320
dc.identifier S2211-1247(15)01310-8
dc.identifier.uri https://hdl.handle.net/10161/12637
dc.description.abstract Calcium signaling has long been associated with key events of immunity, including chemotaxis, phagocytosis, and activation. However, imaging and manipulation of calcium flux in motile immune cells in live animals remain challenging. Using light-sheet microscopy for in vivo calcium imaging in zebrafish, we observe characteristic patterns of calcium flux triggered by distinct events, including phagocytosis of pathogenic bacteria and migration of neutrophils toward inflammatory stimuli. In contrast to findings from ex vivo studies, we observe enriched calcium influx at the leading edge of migrating neutrophils. To directly manipulate calcium dynamics in vivo, we have developed transgenic lines with cell-specific expression of the mammalian TRPV1 channel, enabling ligand-gated, reversible, and spatiotemporal control of calcium influx. We find that controlled calcium influx can function to help define the neutrophil's leading edge. Cell-specific TRPV1 expression may have broad utility for precise control of calcium dynamics in other immune cell types and organisms.
dc.language eng
dc.publisher Elsevier BV
dc.relation.ispartof Cell Rep
dc.relation.isversionof 10.1016/j.celrep.2015.11.010
dc.subject Animals
dc.subject Animals, Genetically Modified
dc.subject Calcium
dc.subject Calcium Signaling
dc.subject Chemotaxis
dc.subject Microscopy, Fluorescence
dc.subject Neutrophils
dc.subject Rats
dc.subject TRPV Cation Channels
dc.subject Zebrafish
dc.title Direct In Vivo Manipulation and Imaging of Calcium Transients in Neutrophils Identify a Critical Role for Leading-Edge Calcium Flux.
dc.type Journal article
duke.contributor.id Tobin, DM|0544801
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/26673320
pubs.begin-page 2107
pubs.end-page 2117
pubs.issue 10
pubs.organisational-group Basic Science Departments
pubs.organisational-group Duke
pubs.organisational-group Immunology
pubs.organisational-group Molecular Genetics and Microbiology
pubs.organisational-group School of Medicine
pubs.publication-status Published
pubs.volume 13
dc.identifier.eissn 2211-1247
duke.contributor.orcid Tobin, DM|0000-0003-3465-5518


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