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Multiscale photoacoustic tomography using reversibly switchable bacterial phytochrome as a near-infrared photochromic probe.

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Date
2016-01
Authors
Yao, Junjie
Kaberniuk, Andrii A
Li, Lei
Shcherbakova, Daria M
Zhang, Ruiying
Wang, Lidai
Li, Guo
Verkhusha, Vladislav V
Wang, Lihong V
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Abstract
Photoacoustic tomography (PAT) of genetically encoded probes allows for imaging of targeted biological processes deep in tissues with high spatial resolution; however, high background signals from blood can limit the achievable detection sensitivity. Here we describe a reversibly switchable nonfluorescent bacterial phytochrome for use in multiscale photoacoustic imaging, BphP1, with the most red-shifted absorption among genetically encoded probes. BphP1 binds a heme-derived biliverdin chromophore and is reversibly photoconvertible between red and near-infrared light-absorption states. We combined single-wavelength PAT with efficient BphP1 photoswitching, which enabled differential imaging with substantially decreased background signals, enhanced detection sensitivity, increased penetration depth and improved spatial resolution. We monitored tumor growth and metastasis with ∼ 100-μm resolution at depths approaching 10 mm using photoacoustic computed tomography, and we imaged individual cancer cells with a suboptical-diffraction resolution of ∼ 140 nm using photoacoustic microscopy. This technology is promising for biomedical studies at several scales.
Type
Journal article
Subject
Acoustics
Animals
Cell Line, Tumor
Humans
Mice
Phytochrome
Tomography
Permalink
https://hdl.handle.net/10161/12772
Published Version (Please cite this version)
10.1038/nmeth.3656
Publication Info
Yao, Junjie; Kaberniuk, Andrii A; Li, Lei; Shcherbakova, Daria M; Zhang, Ruiying; Wang, Lidai; ... Wang, Lihong V (2016). Multiscale photoacoustic tomography using reversibly switchable bacterial phytochrome as a near-infrared photochromic probe. Nat Methods, 13(1). pp. 67-73. 10.1038/nmeth.3656. Retrieved from https://hdl.handle.net/10161/12772.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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Scholars@Duke

Yao

Junjie Yao

Assistant Professor of Biomedical Engineering
Our mission at PI-Lab is to develop state-of-the-art photoacoustic tomography (PAT) technologies and translate PAT advances into diagnostic and therapeutic applications, especially in functional brain imaging and early cancer theranostics. PAT is the most sensitive modality for imaging rich optical absorption contrast over a wide range of spatial scales at high speed, and is one of the fastest growing biomedical imaging technologies. Using numerous endogenous and exogenous contrasts, PAT can
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