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Multiscale photoacoustic tomography using reversibly switchable bacterial phytochrome as a near-infrared photochromic probe.

dc.contributor.author Kaberniuk, AA
dc.contributor.author Li, G
dc.contributor.author Li, L
dc.contributor.author Shcherbakova, DM
dc.contributor.author Verkhusha, VV
dc.contributor.author Wang, L
dc.contributor.author Wang, LV
dc.contributor.author Yao, Junjie
dc.contributor.author Zhang, R
dc.coverage.spatial United States
dc.date.accessioned 2016-09-14T20:31:28Z
dc.date.issued 2016-01
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/26550774
dc.identifier nmeth.3656
dc.identifier.uri https://hdl.handle.net/10161/12772
dc.description.abstract Photoacoustic tomography (PAT) of genetically encoded probes allows for imaging of targeted biological processes deep in tissues with high spatial resolution; however, high background signals from blood can limit the achievable detection sensitivity. Here we describe a reversibly switchable nonfluorescent bacterial phytochrome for use in multiscale photoacoustic imaging, BphP1, with the most red-shifted absorption among genetically encoded probes. BphP1 binds a heme-derived biliverdin chromophore and is reversibly photoconvertible between red and near-infrared light-absorption states. We combined single-wavelength PAT with efficient BphP1 photoswitching, which enabled differential imaging with substantially decreased background signals, enhanced detection sensitivity, increased penetration depth and improved spatial resolution. We monitored tumor growth and metastasis with ∼ 100-μm resolution at depths approaching 10 mm using photoacoustic computed tomography, and we imaged individual cancer cells with a suboptical-diffraction resolution of ∼ 140 nm using photoacoustic microscopy. This technology is promising for biomedical studies at several scales.
dc.language eng
dc.relation.ispartof Nat Methods
dc.relation.isversionof 10.1038/nmeth.3656
dc.subject Acoustics
dc.subject Animals
dc.subject Cell Line, Tumor
dc.subject Humans
dc.subject Mice
dc.subject Phytochrome
dc.subject Tomography
dc.title Multiscale photoacoustic tomography using reversibly switchable bacterial phytochrome as a near-infrared photochromic probe.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/26550774
pubs.begin-page 67
pubs.end-page 73
pubs.issue 1
pubs.organisational-group Biomedical Engineering
pubs.organisational-group Duke
pubs.organisational-group Pratt School of Engineering
pubs.publication-status Published
pubs.volume 13
dc.identifier.eissn 1548-7105


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