High-speed label-free functional photoacoustic microscopy of mouse brain in action.
Abstract
We present fast functional photoacoustic microscopy (PAM) for three-dimensional high-resolution,
high-speed imaging of the mouse brain, complementary to other imaging modalities.
We implemented a single-wavelength pulse-width-based method with a one-dimensional
imaging rate of 100 kHz to image blood oxygenation with capillary-level resolution.
We applied PAM to image the vascular morphology, blood oxygenation, blood flow and
oxygen metabolism in both resting and stimulated states in the mouse brain.
Type
Journal articleSubject
AnimalsBlood Flow Velocity
Brain
Electric Stimulation
Female
Mice
Microscopy
Photoacoustic Techniques
Reproducibility of Results
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https://hdl.handle.net/10161/12773Published Version (Please cite this version)
10.1038/nmeth.3336Publication Info
(2015). High-speed label-free functional photoacoustic microscopy of mouse brain in action.
Nat Methods, 12(5). pp. 407-410. 10.1038/nmeth.3336. Retrieved from https://hdl.handle.net/10161/12773.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Junjie Yao
Associate Professor of Biomedical Engineering
Our mission at PI-Lab is to develop state-of-the-art photoacoustic tomography (PAT)
technologies and translate PAT advances into diagnostic and therapeutic applications,
especially in functional brain imaging and early cancer theranostics. PAT is the most
sensitive modality for imaging rich optical absorption contrast over a wide range
of spatial scales at high speed, and is one of the fastest growing biomedical imaging
technologies. Using numerous endogenous and exogenous contrasts, PAT can
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