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Aneuploidy Tolerance in a Polyploid Organ

dc.contributor.advisor Fox, Donald T
dc.contributor.advisor Lew, Daniel J Schoenfelder, Kevin Paul 2016-09-29T14:39:54Z 2018-08-08T08:17:08Z 2016
dc.description.abstract <p>Endopolyploid cells (hereafter - polyploid cells), which contain whole genome duplications in an otherwise diploid organism, play vital roles in development and physiology of diverse organs such as our heart and liver. Polyploidy is also observed with high frequency in many tumors, and division of such cells frequently creates aneuploidy (chromosomal imbalances), a hallmark of cancer. Despite its frequent occurrence and association with aneuploidy, little is known about the specific role that polyploidy plays in diverse contexts. Using a new model tissue, the Drosophila rectal papilla, we sought to uncover connections between polyploidy and aneuploidy during organ development. Our lab previously discovered that the papillar cells of the Drosophila hindgut undergo developmentally programmed polyploid cell divisions, and that these polyploid cell divisions are highly error-prone. Time-lapse studies of polyploid mitosis revealed that the papillar cells undergo a high percentage of tripolar anaphase, which causes extreme aneuploidy. Despite this massive chromosome imbalance, we found the tripolar daughter cells are viable and support normal organ development and function, suggesting acquiring extra genome sets enables a cell to tolerate the genomic alterations incurred by aneuploidy. We further extended these findings by seeking mechanisms by which the papillar cells tolerated this resultant aneuploidy.</p>
dc.subject Developmental biology
dc.subject Cellular biology
dc.subject Biology
dc.subject Aneuploid
dc.subject Drosophila
dc.subject Multipolar
dc.subject papilla
dc.subject Polyploid
dc.subject rectal
dc.title Aneuploidy Tolerance in a Polyploid Organ
dc.type Dissertation
dc.department Genetics and Genomics
duke.embargo.months 22

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