Imaging Polarization in Budding Yeast.
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We describe methods for live-cell imaging of yeast cells that we have exploited to image yeast polarity establishment. As a rare event occurring on a fast time-scale, imaging polarization involves a trade-off between spatiotemporal resolution and long-term imaging without excessive phototoxicity. By synchronizing cells in a way that increases resistance to photodamage, we discovered unexpected aspects of polarization including transient intermediates with more than one polarity cluster, oscillatory clustering of polarity factors, and mobile "wandering" polarity sites.
Published Version (Please cite this version)10.1007/978-1-4939-3480-5_2
Publication InfoJohnson, SA; Lew, Daniel J; McClure, AW; & Wu, CF (2016). Imaging Polarization in Budding Yeast. Methods Mol Biol, 1407. pp. 13-23. 10.1007/978-1-4939-3480-5_2. Retrieved from https://hdl.handle.net/10161/13037.
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James B. Duke Professor of Pharmacology and Cancer Biology
Our research interests focus on the control of cell polarity. Cell polarity is a nearly universal feature of eukaryotic cells. A polarized cell usually has a single, clear axis of asymmetry: a “front” and a “back”. In the past several years it has become apparent that the highly conserved Rho-family GTPase Cdc42, first discovered in yeast, is a component of a master pathway, employed time and again to promote polarity in different contexts.