Systematic functional analysis of kinases in the fungal pathogen Cryptococcus neoformans.
Repository Usage Stats
Cryptococcus neoformans is the leading cause of death by fungal meningoencephalitis; however, treatment options remain limited. Here we report the construction of 264 signature-tagged gene-deletion strains for 129 putative kinases, and examine their phenotypic traits under 30 distinct in vitro growth conditions and in two different hosts (insect larvae and mice). Clustering analysis of in vitro phenotypic traits indicates that several of these kinases have roles in known signalling pathways, and identifies hitherto uncharacterized signalling cascades. Virulence assays in the insect and mouse models provide evidence of pathogenicity-related roles for 63 kinases involved in the following biological categories: growth and cell cycle, nutrient metabolism, stress response and adaptation, cell signalling, cell polarity and morphology, vacuole trafficking, transfer RNA (tRNA) modification and other functions. Our study provides insights into the pathobiological signalling circuitry of C. neoformans and identifies potential anticryptococcal or antifungal drug targets.
Published Version (Please cite this version)10.1038/ncomms12766
Publication InfoLee, KT; So, YS; Yang, DH; Jung, KW; Choi, J; Lee, DG; ... Bahn, YS (2016). Systematic functional analysis of kinases in the fungal pathogen Cryptococcus neoformans. Nat Commun, 7. pp. 12766. 10.1038/ncomms12766. Retrieved from https://hdl.handle.net/10161/13057.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
More InfoShow full item record
Chair, Department of Molecular Genetics and Microbiology
Signal transduction cascades regulating development and virulence of microorganisms Our research focuses on how cells sense their environment and communicate with other cells. We employ genetic and biochemical approaches to study two divergent single-celled eukaryotic organisms, the yeast Saccharomyces cerevisiae and the pathogenic fungus Cryptococcus neoformans. These organisms both grow as budding yeasts and appear quite similar, yet they have been diverging ov