Capturing and Manipulating Activated Neuronal Ensembles with CANE Delineates a Hypothalamic Social-Fear Circuit
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© 2016 Elsevier Inc.We developed a technology (capturing activated neuronal ensembles [CANE]) to label, manipulate, and transsynaptically trace neural circuits that are transiently activated in behavioral contexts with high efficiency and temporal precision. CANE consists of a knockin mouse and engineered viruses designed to specifically infect activated neurons. Using CANE, we selectively labeled neurons that were activated by either fearful or aggressive social encounters in a hypothalamic subnucleus previously known as a locus for aggression, and discovered that social-fear and aggression neurons are intermixed but largely distinct. Optogenetic stimulation of CANE-captured social-fear neurons (SFNs) is sufficient to evoke fear-like behaviors in normal social contexts, whereas silencing SFNs resulted in reduced social avoidance. CANE-based mapping of axonal projections and presynaptic inputs to SFNs further revealed a highly distributed and recurrent neural network. CANE is a broadly applicable technology for dissecting causality and connectivity of spatially intermingled but functionally distinct ensembles.
Published Version (Please cite this version)10.1016/j.neuron.2016.10.015
Publication InfoFu, M; Han, BX; Leavitt, AD; Lu, Jianfeng; Rodriguez, E; Sakurai, K; ... Zhao, S (2016). Capturing and Manipulating Activated Neuronal Ensembles with CANE Delineates a Hypothalamic Social-Fear Circuit. Neuron, 92(4). pp. 739-753. 10.1016/j.neuron.2016.10.015. Retrieved from http://hdl.handle.net/10161/13276.
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Associate Professor of Mathematics
Jianfeng Lu is an applied mathematician interested in mathematical analysis and algorithm development for problems from computational physics, theoretical chemistry, materials science and other related fields.More specifically, his current research focuses include:Electronic structure and many body problems; quantum molecular dynamics; multiscale modeling and analysis; rare events and sampling techniques.
Morris N. Broad Distinguished Professor
My lab studies neural circuit basis of sensory perception and motor action.Specifically we are interested in determining functions as well as mapping the meso-scale connectivity of neural circuits involved in (1) voluntary movements; (2) touch and pain sensation; and (3) social interactions. We use a combination of genetic, viral, electrophysiology, and in vivo imaging (in free-moving animals) techniques to study these questions.
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