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1,2-Diacylglycerol choline phosphotransferase catalyzes the final step in the unique Treponema denticola phosphatidylcholine biosynthesis pathway.

dc.contributor.author Castillo-Ramirez, S
dc.contributor.author Christopher, J Fenno
dc.contributor.author Córdoba-Castro, LA
dc.contributor.author Geiger, O
dc.contributor.author Goetting-Minesky, M Paula
dc.contributor.author Guan, Ziqiang
dc.contributor.author López-Lara, IM
dc.contributor.author Sohlenkamp, C
dc.contributor.author Vences-Guzmán, MÁ
dc.coverage.spatial England
dc.date.accessioned 2017-01-01T14:14:11Z
dc.date.issued 2016-12-23
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/28009086
dc.identifier.uri http://hdl.handle.net/10161/13290
dc.description.abstract Treponema denticola synthesizes phosphatidylcholine through a licCA-dependent CDP-choline pathway identified only in the genus Treponema. However, the mechanism of conversion of CDP-choline to phosphatidylcholine remained unclear. We report here characterization of TDE0021 (herein designated cpt) encoding a 1,2-diacylglycerol choline phosphotransferase homologous to choline phosphotransferases that catalyze the final step of the highly conserved Kennedy pathway for phosphatidylcholine synthesis in eukaryotes. T. denticola Cpt catalyzed in vitro phosphatidylcholine formation from CDP-choline and diacylglycerol, and full activity required divalent manganese. Allelic replacement mutagenesis of cpt in T. denticola resulted in abrogation of phosphatidylcholine synthesis. T. denticola Cpt complemented a Saccharomyces cerevisiae CPT1 mutant, and expression of the entire T. denticola LicCA-Cpt pathway in E. coli resulted in phosphatidylcholine biosynthesis. Our findings show that T. denticola possesses a unique phosphatidylcholine synthesis pathway combining conserved prokaryotic choline kinase and CTP:phosphocholine cytidylyltransferase activities with a 1,2-diacylglycerol choline phosphotransferase that is common in eukaryotes. Other than in a subset of mammalian host-associated Treponema that includes T. pallidum, this pathway is found in neither bacteria nor Archaea. Molecular dating analysis of the Cpt gene family suggests that a horizontal gene transfer event introduced this gene into an ancestral Treponema well after its divergence from other spirochetes.
dc.language eng
dc.relation.ispartof Mol Microbiol
dc.relation.isversionof 10.1111/mmi.13596
dc.title 1,2-Diacylglycerol choline phosphotransferase catalyzes the final step in the unique Treponema denticola phosphatidylcholine biosynthesis pathway.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/28009086
pubs.organisational-group Basic Science Departments
pubs.organisational-group Biochemistry
pubs.organisational-group Duke
pubs.organisational-group School of Medicine
pubs.publication-status Published online
dc.identifier.eissn 1365-2958


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