Polymorphisms in Fibronectin Binding Proteins A and B among Staphylococcus aureus Bloodstream Isolates Are Not Associated with Arthroplasty Infection.
Abstract
BACKGROUND: Nonsynonymous single nucleotide polymorphisms (SNPs) in fibronectin binding
protein A (fnbA) of Staphylococcus aureus are associated with cardiac device infections.
However, the role of fnbA SNPs in S. aureus arthroplasty infection is unknown. METHODS:
Bloodstream S. aureus isolates from a derivation cohort of patients at a single U.S.
medical center with S. aureus bacteremia (SAB) and prosthetic hip or knee arthroplasties
that were infected (PJI, n = 27) or uninfected (PJU, n = 43) underwent sequencing
of fnbA and fnbB. A validation cohort of S. aureus bloodstream PJI (n = 12) and PJU
(n = 58) isolates from Germany also underwent fnbA and fnbB sequencing. RESULTS: Overall,
none of the individual fnbA or fnbB SNPs were significantly associated with the PJI
or PJU clinical groups within the derivation cohort. Similarly, none of the individual
fnbA or fnbB SNPs were associated with PJI or PJU when the analysis was restricted
to patients with either early SAB (i.e., bacteremia occurring <1 year after placement
or manipulation of prostheses) or late SAB (i.e., bacteremia >1 year after placement
or manipulation of prostheses). CONCLUSIONS: In contrast to cardiac device infections,
there is no association between nonsynonymous SNPs in fnbA or fnbB of bloodstream
S. aureus isolates and arthroplasty infection. These results suggest that initial
steps leading to S. aureus infection of cardiovascular and orthopedic prostheses may
arise by distinct processes.
Type
Journal articleSubject
Adhesins, BacterialAdult
Aged
Aged, 80 and over
Amino Acid Sequence
Arthroplasty, Replacement, Hip
Arthroplasty, Replacement, Knee
Bacteremia
Biofilms
Female
Gene Expression
Genetic Association Studies
Humans
Male
Middle Aged
Molecular Sequence Data
Polymorphism, Single Nucleotide
Prosthesis-Related Infections
Sequence Analysis, DNA
Staphylococcal Infections
Staphylococcus aureus
Young Adult
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https://hdl.handle.net/10161/13309Published Version (Please cite this version)
10.1371/journal.pone.0141436Publication Info
Eichenberger, Emily M; Thaden, Joshua T; Sharma-Kuinkel, Batu; Park, Lawrence P; Rude,
Thomas H; Ruffin, Felicia; ... Kaasch, Achim J (2015). Polymorphisms in Fibronectin Binding Proteins A and B among Staphylococcus aureus
Bloodstream Isolates Are Not Associated with Arthroplasty Infection. PLoS One, 10(11). pp. e0141436. 10.1371/journal.pone.0141436. Retrieved from https://hdl.handle.net/10161/13309.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Emily Eichenberger
House Staff
Vance Garrison Fowler Jr.
Florence McAlister Distinguished Professor of Medicine
Determinants of Outcome in Patients with Staphylococcus aureus Bacteremia Antibacterial
ResistancePathogenesis of Bacterial Infections Tropical medicine/International Health
Lawrence P Park
Associate Professor in Medicine
Joshua Thomas Thaden
Assistant Professor of Medicine
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